Guides, FAQs, and protocols to help you get the most out of your system
The IsoCode Chip has published a series of comparator assay metrics to both Fluorospot and flow cytometry Intracellular Cytokine Staining. At the same time, Flow cytometry and single-cell RNAseq assays have their merits and we view them as complentary tools to our technology. Single cell secretion profiles are expected to be different from single cell RNAseq assays. Our functional profiling assays quantify the secretion capacity of single cells whereas cytokine profiling by flow cytometry typically relies on artificially disrupting protein transport within the cell. Many publications exists demonstrating poor correlation between single cell RNAseq and actual protein expression. Furthermore, our technology was able to link secretion profiles to patient outcome with statistical significance in several retrospecitive studies, whereas flow cytometry and other bulk assays were not able to. Please see our publications for more information.