Sample Preparation

Uncover the functional subsets of cells and unlock powerful correlative data with our proven and published sample preparation protocols

IsoSpeak Software: See how IsoPlexis’ validated sample preparation protocols make enrichment and stimulation work for you

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Utilize IsoPlexis’ Validated Sample Preparation Protocols Across Many Use Cases

Access the Breadth of Sample Preparation Protocols to Awaken True Functional Immune Biology

Tech Note: Comprehensive Protocol and Data List

Apply various T-cell and other immune cell protocols from the blood and tissue in various immunotherapy types
Apply engineered T and NK cell protocols that meet your needs from pre-infusion to monitoring
See our protocols in vaccines, autoimmune disease & other emerging areas

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Sample Preparation Process: Detail Supporting Successful Use

Step 1 Sample Enrichment

Sample Enrichment: Phenotypes of interest are isolated

Step 2 Cell Staining

Cell Staining: Cells are fluorescently labeled

Step 3 Cell Stimulation

Cell Stimulation: Stimulation to fit your cell type and use case, in accordance with established protocols

Step 4 Run

Automated analysis: Sample suspension is loaded into the IsoCode chips. The IsoLight images the chips for cell detection, incubates, conducts ELISA protocol, and images for signal

Sample Prep: Access Our Protocols

PSI correlative biomarkers in a wide body of work from the tissue to the blood

Achieve critical correlates to in vivo data with our published enrichment, stimulation, and staining protocols throughout immunotherapy types and indications
Access protocols that minimize the impact on the live cells analyzed on the IsoLight System
Review guidelines for the most commonly used cell types and applications
Quickly get started in sample prep using our intuitive and easy-to-use technology, backed by our team of support pros

Explore Our Protocols

Sample Prep: Getting Started
with Key Details

Getting Started with Key Details

Discover the functional cell subsets that make a difference and achieve your project goals
Access key sample prep protocols and helpful guidelines on enrichment, stimulation, and staining
Review published stimulation plans and precedent data connecting to in vivo physiology across research areas
Follow this sample prep methodology to quickly gather data and ensure success in each run

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