Our IsoCode technology is very different from common spotted protein microarrays. During our chip manufacturing process, we are using precision microfluidics to flow defined amounts of capture antibodies in precise patterns onto our detection array yielding high-density antibody barcodes.
A typical run yields data from roughly 500-1500 single cells across a panel of 25-35 analytes, for a total of roughly 40,000 data points.
We have empirically determined that data obtained from as little as 200 single cells is generally sufficient for functional profiling. However, we recommend at least 500 single cells per chip in order to capture the full heterogeneity of single-cell responses.
Overall, we have about a 20-30% inter-assay variability, which positions our assay between the gold standards Flow-ICS and ELISPOT in terms of variability.
Yes, we have performed extensive testing to determine technical reproducibility.
The IsoCode Chip has published a series of comparator assay metrics to both Fluorospot and flow cytometry Intracellular Cytokine Staining. At the same time, Flow cytometry and single-cell RNAseq assays have their merits and we view them as complentary tools to our technology. Single cell secretion profiles are expected to be different from single cell RNAseq assays. Our functional profiling assays quantify the secretion capacity of single cells whereas cytokine profiling by flow cytometry typically relies on artificially disrupting protein transport within the cell. Many publications exists demonstrating poor correlation between single cell RNAseq and actual protein expression. Furthermore, our technology was able to link secretion profiles to patient outcome with statistical significance in several retrospecitive studies, whereas flow cytometry and other bulk assays were not able to. Please see our publications for more information.
Both the IsoLight and IsoSpark deliver automated readouts into IsoSpeak Software. Watch the technology overview webinar to see our automated end-to-end workflow
Featured Speaker: Peter Tsiompilkas, MS
MS, VP, or Product R&D, IsoPlexis
Technology Webinar: Getting Started with the IsoLight System: How it Works to Automate Your Workflow and Get You Data
Our award-winning single-cell system delivers what you need the most across the board: high-quality results, while saving time and resources
IsoLight automates each step: from imaging, to incubation, to reagents and washing
You’re aware of each step throughout the process, thanks to the intuitive user interface, and the LED beams to let you know project status
Learn more about our award-winning IsoLight system
- Answers in days, not weeks with advanced fluidics and integrated software
- Built-in quality controls, platform wide
- Parallel chip processing to batch experiments, conditions, controls, etc.
- Comprehensive data exploration with publication quality graph export
Prepare your samples using one of our highly validated protocols then let IsoLight do the rest.
- Turnkey enrichment, stimulation, and staining protocols for a variety of critical cell types and biological scenarios
- Automated, parallelized sample processing, data generation, and result visualization including:
- Live cell separation and incubation
- Cell imaging
- Proteomic imaging
- Data analysis and visualization
- Statistical comparisons