IsoCode Single Cell Polyfunctional Strength: TCR-T Protocol
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This SOP outlines the standard method for thawing and culturing of human TCR-T cells only and may not be valid for other species or cell types.
In this Protocol we:
- Day 1:
Cryopreserved TCR-T cells are thawed and cultured overnight in the presence of IL-2.
- Day 2:
Enrichment, Staining and Antigen-Stimulation of CD8+ and/or CD4+ T cells for 20* hours.
- Day 3:
Loading of TCR-T cells onto IsoCode chip*.