IsoCode Single Cell Polyfunctional Strength: TCR-T Protocol

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Protocol Overview

This SOP outlines the standard method for thawing and culturing of human TCR-T cells only and may not be valid for other species or cell types.

In this Protocol we discuss:
  • Day 1:
    Cryopreserved TCR-T cells are thawed and cultured overnight in the presence of IL-2.
  • Day 2:
    Enrichment, Staining and Antigen-Stimulation of CD8+ and/or CD4+ T cells for 20* hours.
  • Day 3:
    Loading of TCR-T cells onto IsoCode chip*.
2019-08-08T18:27:48-04:00