IsoCode Single Cell Polyfunctional Strength: TCR-T Protocol

Get the most out of our system to rapidly generate useful data that relates to in vivo immune activity.

Your Asset is Ready
Your Protocol is now ready to download.

Protocol Overview

This SOP outlines the standard method for thawing and culturing of human TCR-T cells only and may not be valid for other species or cell types.

In this Protocol we:
  • Day 1:
    Cryopreserved TCR-T cells are thawed and cultured overnight in the presence of IL-2.
  • Day 2:
    Enrichment, Staining and Antigen-Stimulation of CD8+ and/or CD4+ T cells for 20* hours.
  • Day 3:
    Loading of TCR-T cells onto IsoCode chip*.