IsoCode Single Cell Polyfunctional Strength: TCR-T Protocol

Get the most out of our system to rapidly generate useful data that relates to in vivo immune activity.

Your data is in safe hands: we'll never sell it to third parties and you may always unsubscribe. Privacy Policy.

Protocol Overview

This SOP outlines the standard method for thawing and culturing of human TCR-T cells only and may not be valid for other species or cell types.

In this Protocol we discuss:
  • Day 1:
    Cryopreserved TCR-T cells are thawed and cultured overnight in the presence of IL-2.
  • Day 2:
    Enrichment, Staining and Antigen-Stimulation of CD8+ and/or CD4+ T cells for 20* hours.
  • Day 3:
    Loading of TCR-T cells onto IsoCode chip*.