This SOP outlines the standard method for thawing and culturing of human CAR-T cells only and may not be valid for other species or cell types.
In this Protocol we:
- Day 1:
Cryopreserved cells are thawed and cultured overnight in the presence of IL-2.
- Day 2:
Enrichment and Stimulation of CD4+ and/or CD8+ T cells for 24 hours.
- Day 3:
Staining and Loading of CAR-T cells onto IsoCode chip