IsoCode Single Cell Polyfunctional Strength: PBMC Protocol

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Protocol Overview

This SOP outlines the standard method for thawing and culturing of human CAR-T cells only and may not be valid for other species or cell types.

In this we discuss:
  • Day 1:
    Cryopreserved cells are thawed and cultured overnight in the presence of IL-2.
  • Day 2:
    Enrichment and Stimulation of CD4+ and/or CD8+ T cells for 24 hours.
  • Day 3:
    Staining and Loading of CAR-T cells onto IsoCode chip