IsoCode Single Cell Polyfunctional Strength: PBMC Protocol
Get the most out of our system to rapidly generate useful data that relates to in vivo immune activity.
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This SOP outlines the standard method for thawing and culturing of human CAR-T cells only and may not be valid for other species or cell types.
In this Protocol we:
- Day 1:
Cryopreserved cells are thawed and cultured overnight in the presence of IL-2.
- Day 2:
Enrichment and Stimulation of CD4+ and/or CD8+ T cells for 24 hours.
- Day 3:
Staining and Loading of CAR-T cells onto IsoCode chip