IsoCode Single Cell Polyfunctional Strength: NK Cell Protocol
Get the most out of our system to rapidly generate useful data that relates to in vivo immune activity.
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This SOP outlines the standard method for thawing and culturing of human CAR-T cells only and may not be valid for other species or cell types.
In this Protocol we:
- Day 1:
Cryopreserved cells are thawed and cultured overnight in the presence of IL-2 (10 ng/mL) at a density of 1-5 x 106 mL.
- Day 2:
Enrichment and Staining of Natural Killer (NK) cells. Stimulation and Loading of NK cells onto IsoCode chip.