This SOP outlines the standard method for thawing and culturing of human CAR-T cells only and may not be valid for other species or cell types.
In this Protocol we:
- Day 1:
Cryopreserved cells are thawed and cultured overnight in the presence of IL-2 (10 ng/mL) at a density of 1-5 x 106 mL.
- Day 2:
Enrichment and Staining of Natural Killer (NK) cells. Stimulation and Loading of NK cells onto IsoCode chip.