IsoCode Single Cell Polyfunctional Strength: CAR-T Protocol

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Protocol Overview

This SOP outlines the standard method for thawing and culturing of human CAR-T cells only and may not be valid for other species or cell types.

In this Protocol we discuss:
  • Day 1: Cryopreserved CAR-T cells are thawed and cultured overnight in the presence of IL-2. Cryopreserved APC target cell lines are thawed, cultured and passaged several days prior to beginning the experiment.
  • Day 2: Enrichment, Staining and Antigen-Stimulation of CD4+ and/or CD8+ T cells for 20* hours.
  • Day 1: Loading of CAR-T cells onto IsoCode chip*.