IsoCode Single Cell Polyfunctional Strength: Bispecific Protocol
Get the most out of our system to rapidly generate useful data that relates to in vivo immune activity.
This SOP outlines the standard method for thawing and culturing of human PBMCs or purified T cells only and may not be valid for other species or cell types.
In this Protocol we:
- Day 1:
Cryopreserved T cells are thawed and cultured overnight in the presence of IL-2.
- Day 2:
Enrichment and Antigen-Stimulation of CD4+ and/or CD8+ T cells for 36-48* hours.
- Day 3:
Staining and Loading of T cells onto IsoCode chip*.