IsoCode Single Cell Innate & Myeloid: Monocyte Protocol

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Protocol Overview

This SOP outlines the standard method for thawing and culturing of human CAR-T cells only and may not be valid for other species or cell types.

In this Protocol we:
  • Day 1:
    Cryopreserved cells are thawed and cultured overnight in complete RPMI media.
  • Day 2:
    Enrichment and Stimulation of Monocytes for 24 hours.
  • Day 3:
    Staining and Loading of Monocytes onto IsoCode chip*.
2019-08-08T18:24:32+00:00