This SOP outlines the standard method for thawing and culturing of human CAR-T cells only and may not be valid for other species or cell types.
In this Protocol we:
- Day 1:
Cryopreserved cells are thawed and cultured overnight in complete RPMI media.
- Day 2:
Enrichment and Stimulation of Monocytes for 24 hours.
- Day 3:
Staining and Loading of Monocytes onto IsoCode chip*.