Use IsoSpeak for automated on-site analysis and advanced, functional, single cell mapping:
Visualize: Make sense of complex single-cell data with intuitive and meaningful visualizations automatically generated for you.
Answer: Connect the dots and get answers faster, with detailed informatics & statistics on cellular potency and durability, all at your fingertips.
Organize: Save time with the latest in-software sample annotations & project organization for immunotherapy workflows.
Dominant Functional Group
The Dominant Functional Group mapping displays a color-coded visualization of data points based on the cytokine profile being secreted. These include inflammatory, regulatory, chemoattractive, stimulatory, and effector cytokine profiles. Non-secretors are displayed in grey.
Utilize t-SNE to Differentiate Cellular Fitness by Cytokine Subsets
IsoPlexis’ t-SNE functional graphs plot cells by differentiating them based on their greatest cytokine-based functional differences.
IsoSpeak can visualize stratification differences driving responders vs. non-responders as shown in 40+ precedent data sets.
Visualized within the same functional plot, the t-SNE graph uncovers functional sources of the cellular biomarkers of response.
Multi-functional cells (also termed polyfunctional cells) secreting multiple cytokines are upregulated in responders.
IsoSpeak reveals functional biological drivers in the various cytokines.
Click to explore 3D visualization types:
- Donor Groups
- Dominant Functional Group
3D t-SNE cell mapping
Polyfunctional Strength Index (PSI™)
Single-cell polyfunctional heat map
Percentage of polyfunctional cells
Individual cytokine intensities secreted by single cells
Polyfunctional Activity Topography (PAT) PCA
Toggle which data subsets are visualized and make customizations in the intuitive dashboard
The single cell cytokine mapping t-SNE provides a 3D visualization of single-cell functional cytokines and a high dimensional mapping of the unique polyfunctional cell subsets.
Polyfunctional Strength Index (PSI) aggregates all single-cell, multi-dimensional secretions from a sample into a single index. The readout combines the polyfunctionality of a sample (frequency of cells secreting multiple cytokines) with the signal intensities for each single cell across the secreted cytokines of the sample. The displayed index is color-coded to show the contribution from different categories of cytokines (e.g., effector vs. stimulatory cytokines).
Dominant sub groups will emerge in PAT PCA graphs, representing significant multi-functional subsets driving the overall response.
Use Heterogeneity Heat Maps to uncover the critical cell subpopulations that exist only in the condition/group of interest.
IsoSpeak’s push-button user interface and advanced automation allows users to visualize, target, and utilize data from direct, functional cytokine profiling of single cells for the first time:
Fully automated, parallelized image processing handles data extraction and quality control
Sample annotation & project organization for immunotherapy workflows
End to end informatics and statistics that enables comparison & correlation across your experiments
IsoSpeak Data Analysis and Visualization
Learn how IsoSpeak empowers users with the ability to explore their high dimensional functional data interactively.
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