ISOSPEAK SOFTWARE

Data Visualization Software for IsoLight

Visualize how each immune cell is functionally communicating & contributing to orchestrating response.

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OVERVIEW

IsoSpeak Reveals Immune Insight

Use IsoSpeak for automated on-site analysis and advanced, functional, single cell mapping:

Visualize: Make sense of complex single-cell data with intuitive and meaningful visualizations automatically generated for you.
Answer: Connect the dots and get answers faster, with detailed informatics & statistics on cellular potency and durability, all at your fingertips.
Organize: Save time with the latest in-software sample annotations & project organization for immunotherapy workflows.

IsoSpeak Data Visualization

Push-Button Informatics to Accelerate Your Workflows

t-SNE

Donor Groups

Donor Group cell mapping allows users to stratify data points from samples by responders (orange) versus non-responders (blue).

Next: Click on Polyfunctionality to discover what the differences are between Responders and Non-responders

Polyfunctionality

The Polyfunctionality cell mapping displays data points based on the degree of polyfunctionality of the samples. The darker orange indicates a higher number of non-redundant cytokines secreted per single-cell.

Next: Click Dominant Functional Group to discover which cytokines are being secreting functionally by this subset

Dominant Functional Group

The Dominant Functional Group mapping displays a color-coded visualization of data points based on the cytokine profile being secreted. These include inflammatory, regulatory, chemoattractive, stimulatory, and effector cytokine profiles. Non-secretors are displayed in grey.

Next: These are the biological drivers (cytokines) that are being secreted by these unique subsets. In the expandable panels below you can see how we can visualize the significant of these subsets

Utilize t-SNE to Differentiate Cellular Fitness by Cytokine Subsets

IsoPlexis’ t-SNE functional graphs plot cells by differentiating them based on their greatest cytokine-based functional differences.

IsoSpeak can visualize stratification differences driving responders vs. non-responders as shown in 40+ precedent data sets.

Visualized within the same functional plot, the t-SNE graph uncovers functional sources of the cellular biomarkers of response.

Multi-functional cells (also termed polyfunctional cells) secreting multiple cytokines are upregulated in responders.

IsoSpeak reveals functional biological drivers in the various cytokines.

Click to explore 3D visualization types:

Donor Groups
Polyfunctionality
Dominant Functional Group

Visualize Your Data

Unique Visualization Options

A unique set of visualizations shows the impact of polyfunctionality and what it’s made up of, including:

3D t-SNE cell mapping
Polyfunctional Strength Index (PSI™)
Single-cell polyfunctional heat map
Percentage of polyfunctional cells
Individual cytokine intensities secreted by single cells
Polyfunctional Activity Topography (PAT) PCA
Toggle which data subsets are visualized and make customizations in the intuitive dashboard

Utilize t-SNE to differentiate cellular fitness by cytokine subset

The single cell cytokine mapping t-SNE provides a 3D visualization of single-cell functional cytokines and a high dimensional mapping of the unique polyfunctional cell subsets.

Discover cell product potency differences with polyfunctional strength (PSI) cytokine signatures

Polyfunctional Strength Index (PSI) aggregates all single-cell, multi-dimensional secretions from a sample into a single index. The readout combines the polyfunctionality of a sample (frequency of cells secreting multiple cytokines) with the signal intensities for each single cell across the secreted cytokines of the sample. The displayed index is color-coded to show the contribution from different categories of cytokines (e.g., effector vs. stimulatory cytokines).

Stratify donor/patient response by cellular cytokine signature using polyfunctional PCA

Dominant sub groups will emerge in PAT PCA graphs, representing significant multi-functional subsets driving the overall response.

Group bioprocessing correlations by potent polyfunctional cells

Use Heterogeneity Heat Maps to uncover the critical cell subpopulations that exist only in the condition/group of interest.

ISOSPEAK DATA VISUALIZATION

Gain Data Mastery Quickly With an Easy to Use Interface

IsoSpeak’s push-button user interface and advanced automation allows users to visualize, target, and utilize data from direct, functional cytokine profiling of single cells for the first time:

Fully automated, parallelized image processing handles data extraction and quality control
Sample annotation & project organization for immunotherapy workflows
End to end informatics and statistics that enables comparison & correlation across your experiments

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Download Manual

Learn by Watching

IsoPlexis Video Course

18 CHAPTERS

IsoSpeak Data Analysis and Visualization

Learn how IsoSpeak empowers users with the ability to explore their high dimensional functional data interactively.

Explore by Chapter

Chapter 1: Getting Started 0:50
Chapter 2: Sample Validation using IsoQ Score 0:57
Chapter 3: Analytics Interface Overview 1:01
Chapter 4: Analytics Interface: Biomarker Analysis & Data Vizualization 1:07
Chapter 5: Single Cell PAT-PCA 1:22
Chapter 6: Single-Cell Polyfunctional Heatmap 1:11
Chapter 7: Single-Cell Polyfunctional Overview 1:12
Chapter 8: Single-Cell Polyfunctional Strength Index (PSI) 1:21
Chapter 9: Single-Cell Polyfunctional Strength Index Individual Cytokine Contribution 1:17
Chapter 10: Mapping 3D t-SNE 3:20
Chapter 11: Generating a 3D UMAP 2:21
Chapter 12A: Single-Cell Intracellular Proteome Visualizations, Part A: Signal Intensity Heatmap 1:23
Chapter 12B: Single-Cell Intracellular Proteome Visualizations, Part B: Signal Intensity Violin Plot 1:23
Chapter 12C: Single-Cell Intracellular Proteome Visualizations, Part C: 2D t-SNE 1:18
Chapter 12D: Single-Cell Intracellular Proteome Visualizations, Part D: Correlation Chord Diagram 1:04
Chapter 13: Exporting Data 0:56
Chapter Appendix: Field Guide - Scientific Support 5:06
Chapter Appendix: Field Guide - Analyzing Your Data 5:17

Case Studies & Research

Product	Product Code
IsoLight System	ISOLIGHT-1000-1
IsoSpeak Software	ISOSPEAK-1000-1
IsoLight Service Agreement - 1 Year	SVCISL-1000-1
IsoLight Service Agreement - 2 Years	SVCISL-1000-2
IsoLight Service Agreement - 3 Years	SVCISL-1000-3