Use IsoSpeak for automated on-site analysis and advanced, functional, single cell mapping:
Visualize: Make sense of complex single-cell data with intuitive and meaningful visualizations automatically generated for you.
Answer: Connect the dots and get answers faster, with detailed informatics & statistics on cellular potency and durability, all at your fingertips.
Organize: Save time with the latest in-software sample annotations & project organization for immunotherapy workflows.
3D t-SNE cell mapping
Polyfunctional Strength Index (PSI™)
Single-cell polyfunctional heat map
Percentage of polyfunctional cells
Individual cytokine intensities secreted by single cells
Polyfunctional Activity Topography (PAT) PCA
Toggle which data subsets are visualized and make customizations in the intuitive dashboard
The single cell cytokine mapping t-SNE provides a 3D visualization of single-cell functional cytokines and a high dimensional mapping of the unique polyfunctional cell subsets.
Polyfunctional Strength Index (PSI) aggregates all single-cell, multi-dimensional secretions from a sample into a single index. The readout combines the polyfunctionality of a sample (frequency of cells secreting multiple cytokines) with the signal intensities for each single cell across the secreted cytokines of the sample. The displayed index is color-coded to show the contribution from different categories of cytokines (e.g., effector vs. stimulatory cytokines).
Dominant sub groups will emerge in PAT PCA graphs, representing significant multi-functional subsets driving the overall response.
Use Heterogeneity Heat Maps to uncover the critical cell subpopulations that exist only in the condition/group of interest.
IsoSpeak’s push-button user interface and advanced automation allows users to visualize, target, and utilize data from direct, functional cytokine profiling of single cells for the first time:
Fully automated, parallelized image processing handles data extraction and quality control
Sample annotation & project organization for immunotherapy workflows
End to end informatics and statistics that enables comparison & correlation across your experiments
13 CHAPTERS
IsoSpeak Data Analysis and Visualization
Learn how IsoSpeak empowers users with the ability to explore their high dimensional functional data interactively.
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| IsoLight System | ISOLIGHT-1000-1 |
| IsoSpeak Software | ISOSPEAK-1000-1 |
| IsoLight Service Agreement - 1 Year | SVCISL-1000-1 |
| IsoLight Service Agreement - 2 Years | SVCISL-1000-2 |
| IsoLight Service Agreement - 3 Years | SVCISL-1000-3 |
