IsoSpeak

IsoSpeak: Data Visualization Software for IsoLight

Visualize how each immune cell is functionally communicating & contributing to orchestrating response

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IsoSpeak

IsoSpeak Reveals Immune Insight

Use IsoSpeak for automated on-site analysis
and advanced, functional, single cell mapping

Visualize: Make sense of complex single-cell data with intuitive and meaningful visualizations automatically generated for you.

Answer: Connect the dots and get answers faster, with detailed informatics & statistics on cellular potency and durability, all at your fingertips.

Organize: Save time with the latest in-software sample annotations & project organization for immunotherapy workflows.

Push-Button Informatics to Accelerate Your Workflows

IsoPlexis Video Course
11 CHAPTERS

IsoSpeak Data Analysis and Visualization

Learn how IsoSpeak empowers users with the ability to explore their high dimensional functional data interactively.

Visualize Your Data

Gain data mastery quickly with an easy to use interface

IsoSpeak’s push-button user interface and advanced automation allows users to visualize, target, and utilize data from direct, functional cytokine profiling of single cells for the first time:
  • Fully automated, parallelized image processing handles data extraction and quality control
  • Sample annotation & project organization for immunotherapy workflows
  • End to end informatics and statistics that enables comparison & correlation across your experiments
Download Manual
IsoSpeak’s push-button user interface and advanced automation allows users to visualize, target, and utilize data from direct, functional cytokine profiling of single cells for the first time:
  • Fully automated, parallelized image processing handles data extraction and quality control
  • Sample annotation & project organization for immunotherapy workflows
  • End to end informatics and statistics that enables comparison & correlation across your experiments
Download Manual
IsoSpeak Software
A unique set of visualizations shows the impact of polyfunctionality and what it’s made up of, including:
  • 3D t-SNE cell mapping

  • Polyfunctional Strength Index (PSI™)

  • Single-cell polyfunctional heat map

  • Percentage of polyfunctional cells
  • Individual cytokine intensities secreted by single cells
  • Polyfunctional Activity Topography (PAT) PCA

  • Toggle which data subsets are visualized and make customizations in the intuitive dashboard
Download Application Note
t-sne
Utilize 3D t-SNE to differentiate cellular fitness by cytokine subset

The single cell cytokine mapping t-SNE provides a 3D visualization of single-cell functional cytokines and a high dimensional mapping of the unique polyfunctional cell subsets.

Utilize t-SNE to differentiate cellular fitness by cytokine subset

The single cell cytokine mapping t-SNE provides a 3D visualization of single-cell functional cytokines and a high dimensional mapping of the unique polyfunctional cell subsets.

t-sne
PSI
Discover cell product potency differences with polyfunctional strength (PSI) cytokine signatures

Polyfunctional Strength Index (PSI) aggregates all single-cell, multi-dimensional secretions from a sample into a single index. The readout combines the polyfunctionality of a sample (frequency of cells secreting multiple cytokines) with the signal intensities for each single cell across the secreted cytokines of the sample. The displayed index is color-coded to show the contribution from different categories of cytokines (e.g., effector vs. stimulatory cytokines).

PSI
Discover cell product potency differences with polyfunctional strength (PSI) cytokine signatures

Polyfunctional Strength Index (PSI) aggregates all single-cell, multi-dimensional secretions from a sample into a single index. The readout combines the polyfunctionality of a sample (frequency of cells secreting multiple cytokines) with the signal intensities for each single cell across the secreted cytokines of the sample. The displayed index is color-coded to show the contribution from different categories of cytokines (e.g., effector vs. stimulatory cytokines).

Stratify donor/patient response by cellular cytokine signature using polyfunctional PCA

Dominant sub groups will emerge in PAT PCA graphs, representing significant multi-functional subsets driving the overall response.

Stratify donor/patient response by cellular cytokine signature using polyfunctional PCA

Dominant sub groups will emerge in PAT PCA graphs, representing significant multi-functional subsets driving the overall response.

Group bioprocessing correlations by potent polyfunctional cells

Use Heterogeneity Heat Maps to uncover the critical cell subpopulations that exist only in the condition/group of interest.

Group bioprocessing correlations by potent polyfunctional cells

Use Heterogeneity Heat Maps to uncover the critical cell subpopulations that exist only in the condition/group of interest.

See the answers clearly and share your insights
  • Powerful and unique immune polyfunction visualizations that simplify complex single-cell data sets
  • Exploration of functional contributions and secretion level down to individual cells with toggle dashboard
  • Automated data exporting tools give publication ready figures & raw data
Browse Literature
Cytokine Intensity

Explore Resources

Identify the Most Potent Cell Subsets in Your Samples

Our award-winning, single-cell system effortlessly delivers the data you need.

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IsoLight

Discover the Difference

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