IsoSpeak Software

Data Visualization Software for IsoLight

Visualize how each immune cell is functionally communicating & contributing to orchestrating response.

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OVERVIEW

IsoSpeak Reveals Immune Insight

Use IsoSpeak for automated on-site analysis and advanced, functional, single cell mapping:

  • Visualize: Make sense of complex single-cell data with intuitive and meaningful visualizations automatically generated for you.
  • Answer: Connect the dots and get answers faster, with detailed informatics & statistics on cellular potency and durability, all at your fingertips.
  • Organize: Save time with the latest in-software sample annotations & project organization for immunotherapy workflows.
ISOSPEAK DATA VISUALIZATION

Push-Button Informatics to Accelerate Your Workflows

3D Visualizations
Donor Groups
Donor Groups

Donor Group cell mapping allows users to stratify data points from samples by responders (orange) versus non-responders (blue).

Polyfunctionality
Polyfunctionality

The Polyfunctionality cell mapping displays data points based on the degree of polyfunctionality of the samples. The darker orange indicates a higher number of non-redundant cytokines secreted per single-cell.

Dominant Functional Group
Dominant Functional Group

The Dominant Functional Group mapping displays a color-coded visualization of data points based on the cytokine profile being secreted. These include inflammatory, regulatory, chemoattractive, stimulatory, and effector cytokine profiles. Non-secretors are displayed in grey.

Feature
Utilize t-SNE to Differentiate Cellular Fitness by Cytokine Subsets

Bruker Cellular Analysis' t-SNE functional graphs plot cells by differentiating them based on their greatest cytokine-based functional differences.

IsoSpeak can visualize stratification differences driving responders vs. non-responders as shown in 40+ precedent data sets.

Visualized within the same functional plot, the t-SNE graph uncovers functional sources of the cellular biomarkers of response.

Multi-functional cells (also termed polyfunctional cells) secreting multiple cytokines are upregulated in responders.

VISUALIZE YOUR DATA

Unique Visualization Options

A unique set of visualizations shows the impact of polyfunctionality and what it’s made up of, including:

  • Fully automated, parallelized image processing handles data extraction and quality control
  • Sample annotation & project organization for immunotherapy workflows
  • End to end informatics and statistics that enables comparison & correlation across your experiments
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Utilize t-SNE to Differentiate Cellular Fitness by Cytokine Subset

The single cell cytokine mapping t-SNE provides a 3D visualization of single-cell functional cytokines and a high dimensional mapping of the unique polyfunctional cell subsets.

Discover Cell Product Potency Differences With Polyfunctional Strength (PSI) Cytokine Signatures

Polyfunctional Strength Index (PSI) aggregates all single-cell, multi-dimensional secretions from a sample into a single index. The readout combines the polyfunctionality of a sample (frequency of cells secreting multiple cytokines) with the signal intensities for each single cell across the secreted cytokines of the sample. The displayed index is color-coded to show the contribution from different categories of cytokines (e.g., effector vs. stimulatory cytokines).

Stratify Donor/Patient Response by Cellular Cytokine Signature Using Polyfunctional PCA

Dominant sub groups will emerge in PAT PCA graphs, representing significant multi-functional subsets driving the overall response.

Group Bioprocessing Correlations by Potent Polyfunctional Cells

Use Heterogeneity Heat Maps to uncover the critical cell subpopulations that exist only in the condition/group of interest.

Download the IsoSpeak Manual
IsoSpeak Data Visualization

Gain Data Mastery Quickly With an Easy to Use Interface

IsoSpeak’s push-button user interface and advanced automation allows users to visualize, target, and utilize data from direct, functional cytokine profiling of single cells for the first time:

  • Fully automated, parallelized image processing handles data extraction and quality control
  • Sample annotation & project organization for immunotherapy workflows
  • End to end informatics and statistics that enables comparison & correlation across your experiments
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Product Specifications
IsoLight System ISOLIGHT-1000-1
IsoSpeak Software ISOSPEAK-1000-1
IsoLight Service Agreement - 1 Year SVCISL-1000-1
IsoLight Service Agreement - 2 Years SVCISL-1000-2
IsoLight Service Agreement - 3 Years SVCISL-1000-3
Ready to Unlock the Phenome?

Case Studies & Research

Product Video
IsoSpeak Data Analysis and Visualization
Product Catalog
Proteomic Barcoding Product Catalog
Guide
IsoSpeak Manual
Guide
IsoSpeak Guide: Generating and Exporting Reports with IsoSpeak
Tech Note
Automated run quality assessment in IsoSpeak, using the IsoQ Score metric