PBMC Protocol

Watch high definition video to review & learn the IsoCode Single Cell Polyfunctional Strength: PBMC Protocol

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CHAPTER 1
1:29
Getting Started
Performing sample preparation for the IsoCode Single Cell Polyfunctional Strength PBMC Protocol
CHAPTER 2
1:14
Coat Culture Plates with Anti CD3
We’ll begin with coating our culture plate with Anti CD3
CHAPTER 3
2:56
Recovery of Cryopreserved Cells
Recovering your cryopreserved cells
CHAPTER 4
1:38
Pre-Sample Enrichment
Preparing your cells for sample enrichment
CHAPTER 5
6:06
CD8 Sample Enrichment
Enriching for CD8 T Cells
CHAPTER 6
5:34
CD4 Sample Enrichment
Enriching for CD4 T Cells
CHAPTER 7
1:00
Preparation of Stimulation Plate
Preparing your culture plate for cell stimulation
CHAPTER 8
2:04
Cell Stimulation
Instructions on how to begin stimulating your cells
CHAPTER 9
0:39
Chip Thaw
Thawing of the IsoCode Chips 30-60 minutes prior to cell loading
CHAPTER 10
4:18
Cell Staining
Staining your stimulated cells
CHAPTER 11
1:46
Chip Loading
Loading your stained and stimulated cells into the thawed IsoCode Chips
CHAPTER Appendix
3:53
Cell Quantification & Viability
Calculating cell quantification and viability.