Getting Started

Learn about how cutting edge work in single cell proteomics is transforming immunotherapy and multiple other research areas

INTRODUCTION 4 TRAINING MODULES

  • Each learning module contains short references that offer an immediate understanding and a deeper learning experience to fit the time you have
  • Our continuing education equips you to apply the latest cutting edge insight in single cell proteomics to your programs today
IsoU

Select a Training Module to Begin

Module 1
Sample Prep
Module 2
Executing the Run
Module 3
Analyze the Data
Module 4
Apply Our Biology
Validated Protocols

MODULE 1 SINGLE CELL SAMPLE PREP

1A: Learn by Watching

Single-Cell IsoCode Chip sample prep protocol videos enable understanding of key areas requiring attention and required materials to enable single-cell data

1B: Learn by Reading

Review the written the single-cell protocols that apply to your data

1C: Reference Past Runs to Prepare

Review our Tech Note with past data runs across multiple research areas to reference & prepare for your specific runs

1A: Learn by Watching

Watch high definition video to review & learn the IsoCode Single Cell Polyfunctional Strength: PBMC Protocol

PBMC Protocol Training Modules

IsoPlexis Video Course
12 CHAPTERS

IsoCode Single Cell Polyfunctional Strength: PBMC Protocol

Follow along with the validated PBMC Sample Preparation Protocol.

Explore by Chapter

CHAPTER 1
1:29
Getting Started
Performing sample preparation for the IsoCode Single Cell Polyfunctional Strength PBMC Protocol
CHAPTER 2
1:14
Coat Culture Plates with Anti CD3
We’ll begin with coating our culture plate with Anti CD3
CHAPTER 3
2:56
Recovery of Cryopreserved Cells
Recovering your cryopreserved cells
CHAPTER 4
1:38
Pre-Sample Enrichment
Preparing your cells for sample enrichment
CHAPTER 5
6:06
CD8 Sample Enrichment
Enriching for CD8 T Cells
CHAPTER 6
5:34
CD4 Sample Enrichment
Enriching for CD4 T Cells
CHAPTER 7
1:00
Preparation of Stimulation Plate
Preparing your culture plate for cell stimulation
CHAPTER 8
2:04
Cell Stimulation
Instructions on how to begin stimulating your cells
CHAPTER 9
0:39
Chip Thaw
Thawing of the IsoCode Chips 30-60 minutes prior to cell loading
CHAPTER 10
4:18
Cell Staining
Staining your stimulated cells
CHAPTER 11
1:46
Chip Loading
Loading your stained and stimulated cells into the thawed IsoCode Chips
CHAPTER Appendix
3:53
Cell Quantification & Viability
Calculating cell quantification and viability.

 CAR-T Protocol Training Modules

IsoPlexis Video Course
12 CHAPTERS

IsoCode Single Cell Polyfunctional Strength: CAR-T Protocol

Follow along with the validated CAR-T Sample Preparation Protocol.

Explore by Chapter

CHAPTER 1
2:01
Getting Started
Performing sample preparation for the IsoCode Single Cell Polyfunctional Strength CAR-T Protocol.
CHAPTER 2
2:54
Recovery of Target Cell Lines
Recovering your cryopreserved target cell lines. Perform at least three days prior to the recovery of your CAR-T cells (Chapter 4).
CHAPTER 3
3:07
Culture of Target Cells
Culturing your target cells.
CHAPTER 4
2:59
Recovery of CAR-T Cells
Recovering your cryopreserved CAR-T cells.
CHAPTER 5
1:44
Pre-Sample Enrichment
Preparing your CAR-T cells for sample enrichment.
CHAPTER 6
7:43
CD8 Sample Enrichment
Enriching the CD8 CAR-T cells.
CHAPTER 7
3:58
Cell Stimulation
Stimulating your CAR-T cells with your target cells.
CHAPTER 8
7:17
Target Cell Depletion
Performing target cell depletion for your CAR-T cells.
CHAPTER 9
4:04
Cell Staining
Staining your stimulated and target cell depleted CAR-T cells.
CHAPTER 10
0:46
Chip Thaw
Thawing your IsoCode chips 30-60 minutes prior to chip loading.
CHAPTER 11
1:57
Chip Loading
Loading your CAR-T cells onto the thawed IsoCode chips.
CHAPTER Appendix
3:53
Cell Quantification & Viability
Calculating cell quantification and viability.

1B: Learn by Reading

Learn and review our validated sample preparation protocol PDFs starting with our IsoCode Single Cell Polyfunctional Strength: PBMC Protocol

PROTOCOL
Protocol
IsoCode Single Cell Polyfunctional Strength: PBMC Protocol

Get the most out of our system to rapidly generate useful data that relates to in vivo immune activity. VIEW RESOURCE >

TECH NOTE
Tech Note
Getting Started with Your Single-Cell Projects by Following Key Sample Preparation Guidelines

Learn how to ensure that your enrichment, stimulation and labeling will allow you to get physiologically relevant in vivo data right away. VIEW RESOURCE >

ADDITIONAL SUPPORT
protocols
Find the protocol that applies to your project

VIEW ALL >

1C: Reference Past Runs to Prepare

Review our Tech Note with past data runs across multiple research areas to reference and prepare for your specific runs

Access the Breadth of Sample Preparation Protocols to Awaken True Functional Immune Biology

True Functional Immune Biology

Access the Breadth of Sample Preparation Protocols to Awaken True Functional Immune Biology

DOWNLOAD

MODULE 2 EXECUTING THE RUN

2A: Learn by Watching

Learn how an IsoLight run works

2B: Learn by Reading

Learn & review an example project run checklist for all elements of the run

2C: Order Chips

Order chips for your IsoLight run

2A: Learn by Watching

Learn how an IsoLight run works

2B: Learn by Reading

Learn & review an example project run checklist for all elements of the run

Project Checklist

2C: Order Chips

Order chips for your IsoLight Run

Chip Order Form

MODULE 3 ANALYZE THE DATA

3A: Learn by Watching

Watch the Software Demonstration
IsoSpeak Data Analysis and Visualization Videos

3B: Learn by Watching Webinar

Overview of utility of data visualizations

3C: Learn by Reading

Review the Tech Note on how to put together a data report

3A: Learn by Watching

Watch the Software Demonstration

 

IsoSpeak Data Analysis and Visualization Videos

IsoPlexis Video Course
11 CHAPTERS

IsoSpeak Data Analysis and Visualization

Learn how IsoSpeak empowers users with the ability to explore their high dimensional functional data interactively.

Explore by Chapter

CHAPTER 1
0:50
Getting Started
Explore your high dimensional functional data with IsoSpeak.
CHAPTER 2
0:57
Sample Validation using IsoQ Score
Analyze and validate cell sample quality using the IsoQ Score.
CHAPTER 3
1:01
Analytics Interface Overview
Navigate the user interface and customization options in IsoSpeak.
CHAPTER 4
1:07
Analytics Interface: Biomarker Analysis & Data Vizualization
Explore the data visualization options available in IsoSpeak.
CHAPTER 5
1:22
Single Cell PAT-PCA
Generate and export a Single Cell PAT-PCA.
CHAPTER 6
1:11
Single-Cell Polyfunctional Heatmap
Generate and export a Single Cell Polyfunctional Heat Map.
CHAPTER 7
1:12
Single-Cell Polyfunctional Overview
Generate and export a Single Cell Polyfunctional Overview.
CHAPTER 8
1:21
Single-Cell Polyfunctional Strength Index (PSI)
Generate and export a Single Cell Polyfunctional Strength Index (PSI).
CHAPTER 9
1:17
Single-Cell Polyfunctional Strength Index Individual Cytokine Contribution
Generate and export a Single Cell Polyfunctional Strength Index (PSI) measuring individual cytokine contributions.
CHAPTER 10
3:20
Mapping 3D t-SNE
Generate and export a single cell polyfunctional 3D t-SNE for single cell mapping.
CHAPTER 11
0:55
Exporting Data
Explore customization options for exporting your data visualizations in IsoSpeak.

3B: Learn by Watching Webinar

Overview of utility data visualizations

 

3C: Learn by Reading

Review the Tech Note on how to put together a data report

Technical Note

MODULE 4 APPLY OUR BIOLOGY

4A: Learn by Watching

Review webinar for insight from single cell cytokine analysis, not available in bulk or flow

4B: Learn by Reading

View our full list of publications and presentations

4C: Keep up with the Latest Data & Presentations

Keep up with our latest releases & events to understand and apply to your research

Validated Protocols

4A: Learn by Watching

Review webinar for insight from single cell cytokine analysis, not available in bulk or flow

Better by Design: Single-Cell Characterization for Enhanced Cell Therapies

 

Biomarker Strategies for Optimizing Checkpoint Immunotherapy Treatment

 

Improving T-Cell Functionality for Adoptive T-Cell Therapy in Metastatic Colon Cancer

 

4B: Learn by Reading

View our full list of publications and presentations

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Publications & Presentations

Cell Engineering & Therapy

Cancer Immunology

Vaccines & Immunology Research

Inflammatory & Autoimmune Disease

Solid Tumor & Oncology

4C: Keep up with the Latest Data & Presentations

Keep up with our latest releases & events to understand and apply to your research

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EVENTS
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NEWS

Get in Touch

Technical Support:
(475) 221-8402
[email protected]

Select Your Research Area to Follow Our Data

Explore by Research Area

Cell & Gene Therapy

Cancer Immunology

Vaccines & Immunology Research

Inflammation & Autoimmune Response

Solid Tumor Signaling