Follow the Data: Optimize vaccines leading to survival/protection and identify correlates with immune polyfunction1

Research Area: Vaccines & immunology research

Development Stage: Optimization

Goal: Identify differences in  polyfunctionality with different vaccine structures

[unex_ce_button id="content_10khx0ih9,column_content_611g46odk" button_text_color="#ffffff" button_font="bold" button_font_size="18px" button_width="auto" button_alignment="right" button_text_spacing="1px" button_bg_color="#29acd8" button_padding="15px 30px 15px 30px" button_border_width="0px" button_border_color="#29acd8" button_border_radius="0px" button_text_hover_color="#ffffff" button_text_spacing_hover="1px" button_bg_hover_color="#9cc9d8" button_border_hover_color="#9cc9d8" button_link="" button_link_type="url" button_link_target="_blank" has_container="" in_column="1"]Request a seminar
to follow our data >[/ce_button]

How our single-cell systems are making a difference

Solution: PSI reveals key differences in vaccine constructs in mouse models

Finding: Identified a highly polyfunctional subset of hepatic CD8 T cells upon immunization and challenge with novel construct in a way that correlated to outcome in mice

Follow the data

Single-cell functional heat map shows unique and highly polyfunctional hepatic CD8+ T-cell subsets, which were induced in a group of mice that received IrPySpz immunization and live PySpz challenge. A fewer percentage of hepatic CD8+ T cells with high polyfunctionality was induced in either naïve mice or IrNSG-immunized mice (which did not confer protection).

These results demonstrate that a group of polyfunctional hepatic CD8+ T cells, having both effector and chemo-attractive functions at a single cell level, may associate with anti-malaria immunity. The multiple markers, which were unique to hepatic CD8+ T cells derived from IrSpz-immunized and partially protected mice, are identified as a co-secretion of MIP-1α, RANTES, IFN-γ, and/or IL-17A, from a single hepatic CD8+ T cell.1

[unex_ce_button id="content_om95el0ye,column_content_g32kkdowi" button_text_color="#424242" button_font="bold" button_font_size="18px" button_width="auto" button_alignment="right" button_text_spacing="1px " button_bg_color="#c9c9c9" button_padding="15px 30px 15px 30px" button_border_width="0px" button_border_color="#c9c9c9" button_border_radius="0px" button_text_hover_color="#424242" button_text_spacing_hover="1px" button_bg_hover_color="#e8e8e8" button_border_hover_color="#e8e8e8" button_link="" button_link_type="url" button_link_target="_blank" has_container="" in_column="1"]Download application note
to learn more >[/ce_button]