Follow the Data: Sensitively detect subtle differences in CAR-T bioprocessing methods to advance decision making1

Research Area: Cell engineering & therapy

Development Stage: Optimization

Goal: Identify T cell cytokine production differences with varied bioprocessing methods

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How our single-cell systems are making a difference

Solution:  IsoPlexis' systems  and flow cytometry provide the complete picture

Finding: Found consistently more robust, polyfunctional response in modified CAR-T manufacturing protocol

Follow the data

More robust polyfunctional response of CD4+ or CD8+ CAR-T cell products CD19 or CD22 with the modified manufacturing method (MM) compared to the original manufacturing method (OM).

Data demonstrates that the modified CD19/CD22 bispecific CAR-T cell manufacturing method (MM), which terminated T cell activation/transduction by culture day 3, resulted in reproducible and robust CAR-T cell production, even in the relatively more sensitive patient cells (left).1

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IsoPlexis finds critical differences

Significantly higher viable TNC, Fold Expansion, Viability, Final CD3% and Transduction Efficiency in products manufactured using the MM compared to OM. Comparable CD4/CD8 ratios across the two methods.

All products using the MM met product release criteria. In addition, final product dose requirements were consistently met by culture day 7 when using the MM, augmenting process efficiency. 1