Maximizing Our System Single-Cell Data by Referencing Precedent Sample Preparation Protocols

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Tech Note

Ensure that enrichment, stimulation, and labeling will result in maximizing the correlative data achieved while minimizing non-specific impact on the cells themselves.

What's Inside

In this Tech Note we discuss:

Published enrichment, stimulation, and staining protocols throughout immunotherapy types and indications help achieve your goals, in multiple publications and presentations

Protocols that minimize impacts on the live cells that are analyzed on the IsoLight platform

Guidelines for most commonly used cell types and applications

A Deeper Look

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Single-Cell Polyfunctional Strength Application (PSI)

PSI consolidates high-dimensional, single-cell protein secretion data into a single metric representing the overall activity of a sample.

It captures two critically relevant factors uniquely: the percentage of polyfunctional cells (single cells secreting two or more cytokines) in a sample and the intensity of all profiled secreted cytokines.

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