Tech Note
Getting Started with Your Single-Cell Projects by Following Key Sample Preparation Guidelines

Learn how to ensure that your enrichment, stimulation and labeling will allow you to get physiologically relevant in vivo data right away.

The IsoLight System: An Overview

IsoPlexis’ IsoLight system employs its IsoCode® chip, a single-cell, highly multiplexed chip consisting of highly multiplexed antibody capture chip from each single cell. The IsoCode technology uniquely addresses the cytokine detection limitations with its ability to measure a large panel of 40+ key secreted proteins from live single cells and to deeply decipher the functional heterogeneity and key functional subsets of responding immune cells among individual patients.

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Getting Started with Your Single-Cell Projects by Following Key Sample Preparation Guidelines

Learn how to ensure that your enrichment, stimulation and labeling will allow you to get physiologically relevant in vivo data right away. The IsoLight System: An Overview IsoPlexis’ IsoLight system employs its IsoCode® chip, a single-cell, highly multiplexed chip consisting of highly multiplexed antibody capture chip from each single cell. The IsoCode technology uniquely addresses the cytokine detection limitations with its ability to measure a large panel of 40+ key secreted proteins from live single cells and to deeply decipher the functional heterogeneity and key functional subsets of responding immune cells among individual patients.

In this Tech Note we discuss:
  • Guidelines on enrichment, stimulation, and staining, the key sample preparation protocols, that will help achieve your project goals
  • Specific stimulation plans that have been published in the past within various IsoPlexis research areas
  • A sample preparation methodology that will allow multiple types of users to begin to achieve data right away, and ensure success in their run