Getting Started with Your Single-Cell Projects by Following Key Sample Preparation Guidelines

Learn how to ensure that your enrichment, stimulation and labeling will allow you to get physiologically relevant in vivo data right away.

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The IsoLight System: An Overview

IsoPlexis’ IsoLight system employs its IsoCode® chip, a single-cell, highly multiplexed chip consisting of highly multiplexed antibody capture chip from each single cell. The IsoCode technology uniquely addresses the cytokine detection limitations with its ability to measure a large panel of 40+ key secreted proteins from live single cells and to deeply decipher the functional heterogeneity and key functional subsets of responding immune cells among individual patients.

In this Tech Note we:
  • Guidelines on enrichment, stimulation, and staining, the key sample preparation protocols, that will help achieve your project goals
  • Specific stimulation plans that have been published in the past within various IsoPlexis research areas
  • A sample preparation methodology that will allow multiple types of users to begin to achieve data right away, and ensure success in their run