Biological and technical variation in IsoLight single-cell proteomics experiments

Tech Note

Biological and technical variation in IsoLight single-cell proteomics experiments

Explore technical and biological sources of variation and effects on single-cell cytokine secretion data on the IsoLight system.

Your data is secure

We keep your personal information safe and do not share it with any third parties. By submitting this form, you agree to receive communications from IsoPlexis and accept our Privacy Policy. You may unsubscribe at any time.

What's Inside

In this Tech Note we discuss:

Replicate consistency: correlation of t-SNE cell subsets

Sample-to-sample correlation: consistency of secretion intensity across all 32 measured cytokines

Sample-to-sample correlations: split view

IsoPlexis_TechNote_Replicate_Variability_rev1.1.indd-1

IsoPlexis_TechNote_Replicate_Variability_rev1.1.indd-2

A Deeper Look

More about this Tech Note

Single-Cell Proteomics Experiments

Technical and biological sources of variation were evaluated for their effects on single-cell cytokine secretion data from the IsoLight platform.

The nearly identical resulting single-cell t-SNE plots and single-cell intensity levels across all four replicates indicate very low levels of variability.

Download this Tech Note to learn more!

Get the Tech Note

Back to the Top