IsoSpeak Interactive Tour
DATA VISUALIZATIONS
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    • Project Info
    • Data Processing
    • Data Overview
    • Data Visualization
    General
    • No Cells
    • Single-Cell
    • Two-Cell
    Conditions
    • Unlabeled
    • CD19
    • NGFR
    Donors
    • Unlabeled
    • D0001
    • D0005
    • D0006
    Donor Groups
    • Unlabeled
    • Non-Responders
    • Responders
    Markers
    • UNLABELED
    • CD4+
    • CD8+
    Cytokines
    • Clear
    • Secreting
    • All
    • CCL-11
    • GM-CSF
    • GRANZYME
    • IFN-G
    • IL-21
    • IL-22
    • IL-4
    • IL-5
    • IL-17A
    • IL-17F
    • IL-1B
    • IL-9
    • IL-10
    • MCP-4
    • MCP-1a
    • MCP-1B
    • PETFERIN
    • RANTES
    • TGF-B1
    • TGF-B1
    • TGF-B1
    • sCD4DL
    • 456
    • 532
    • 635
    • 751
    • 751
    • t-SNE
    • PF Heatmap
    • PF Overview
    • PF Index
    • PAT-PCA
    • PF Groups
    • Signal Distribution
    t-SNE: Donor Groups
    Responders vs. Non-Responders
    Follow the Data

    This demo data set shows six CD19 CAR-T cell products from three responders and three non-responders.

    Key
    • Non-Responders
    • Responders
    t-SNE: Donor Groups
    Responders vs. Non-Responders
    Follow the Data

    This demo data set shows six CD19 CAR-T cell products from three responders and three non-responders.

    There are clear functional differences between the responders and non-responders.

    But what are those differences?

    Find out in the next t-SNE view.

    Key
    • Non-Responders
    • Responders
    t-SNE: Polyfunctionality
    Compare the Degree of Polyfunctionality
    Follow the Data

    We can see that the responders have the presence of highly polyfunctional CAR-T cells.

    These highly polyfunctional cells have correlated to anti-tumor activity, persistence, and durability in a variety of studies.

    What are the biological drivers of this increased polyfunctionality?

    Find out in the next view.

    Key
    • 5+
    • 4
    • 3
    • 2
    • 1
    • 0
    t-SNE: Dominant Functional Group
    Explore the Secreted Cytokine Profile
    Follow the Data

    The highly polyfunctional subsets of CAR-T cells in the responder groups are driven by a variety of cytokines.

    As shown in this view, we can see that the stimulatory, effector, and chemoattractive cytokines play a dominant role.

    Key
    • Inflammatory
    • Regulatory
    • Chemoattractive
    • Stimulatory
    • Effector
    • Non-secretor
    Heatmap
    Single-Cell Polyfunctional Heatmap
    Follow the Data

    Here you can see that the Responders have more polyfunctional cells than the Non-Responders.

    Key
    • 5%
    • 2.5%
    • 1%
    • 0.5%
    • 0.1%
    Heatmap Key
    PF Overview
    Single-Cell Polyfunctional Overview
    Follow the Data

    It is clear that the percentage of polyfuctional cells in the Responders is much higher than in the Non-Responders.

    Key
    • 2 cytokines
    • 3 cytokines
    • 4 cytokines
    • 5+ cytokines
    CD4 & CD8 Markers
    Polyfunctional Overview
    Follow the Data

    It is clear that the percentage of polyfuctional cells in the Responders is much higher than in the Non-Responders

    Key
    • 2 cytokines
    • 3 cytokines
    • 4 cytokines
    • 5+ cytokines
    PSI
    Single-Cell Polyfunctional Strength Index
    Follow the Data

    Responders have higher polyfunctional immune response driven by primarily stimulatory and effector cytokines

    Key
    • Effector
      Granzyme B, IFN-g, MiIP-1a, Perforin, TNF-a, TNF-b
    • Stimulatory
      GM-CSF, IL12, IL-15, IL-2, IL-21, IL-5, IL-8, IL-9
    • Chemoattractive
      CCL-11, IP-10, MIP-1b, RANTES
    • Regulatory
      IL-10, IL-13, IL-22, IL-4, TGF-b1, sCD137, sCD40L
    • Inflammatory
      IL-17A, IL-17F, IL-1b, IL-6, MCP-1, MCP-4
    Key
    • Effector
      Granzyme B, IFN-g, MiIP-1a, Perforin, TNF-a, TNF-b
    • Stimulatory
      GM-CSF, IL12, IL-15, IL-2, IL-21, IL-5, IL-8, IL-9
    • Chemoattractive
      CCL-11, IP-10, MIP-1b, RANTES
    • Regulatory
      IL-10, IL-13, IL-22, IL-4, TGF-b1, sCD137, sCD40L
    • Inflammatory
      IL-17A, IL-17F, IL-1b, IL-6, MCP-1, MCP-4
    PSI
    Single-Cell Polyfunctional Strength Index
    Follow the Data

    Responders have higher polyfunctional immune response driven by primarily stimulatory and effector cytokines

    Key
    • Effector
      Granzyme B, IFN-g, MiIP-1a, Perforin, TNF-a, TNF-b
    • Stimulatory
      GM-CSF, IL12, IL-15, IL-2, IL-21, IL-5, IL-8, IL-9
    • Chemoattractive
      CCL-11, IP-10, MIP-1b, RANTES
    • Regulatory
      IL-10, IL-13, IL-22, IL-4, TGF-b1, sCD137, sCD40L
    • Inflammatory
      IL-17A, IL-17F, IL-1b, IL-6, MCP-1, MCP-4
    Secreting vs. All
    Single-Cell Polyfunctional Strength Index
    Follow the Data

    Responders have higher polyfunctional immune response driven by primarily stimulatory and effector cytokines

    Secreting vs. All
    Single-Cell Polyfunctional Strength Index
    Follow the Data

    The PSI metric helps you predict that the patients in Therapy 1 are going to respond better to this particular therapy than Therapy 2 patients.