IsoSpeak Interactive Tour
DATA VISUALIZATIONS
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  • Project Info
  • Data Processing
  • Data Overview
  • Data Visualization
General
  • No Cells
  • Single-Cell
  • Two-Cell
Conditions
  • Unlabeled
  • CD19
  • NGFR
Donors
  • Unlabeled
  • D0001
  • D0005
  • D0006
Donor Groups
  • Unlabeled
  • Non-Responders
  • Responders
Markers
  • UNLABELED
  • CD4+
  • CD8+
Cytokines
  • Clear
  • Secreting
  • All
  • CCL-11
  • GM-CSF
  • GRANZYME
  • IFN-G
  • IL-21
  • IL-22
  • IL-4
  • IL-5
  • IL-17A
  • IL-17F
  • IL-1B
  • IL-9
  • IL-10
  • MCP-4
  • MCP-1a
  • MCP-1B
  • PETFERIN
  • RANTES
  • TGF-B1
  • TGF-B1
  • TGF-B1
  • sCD4DL
  • 456
  • 532
  • 635
  • 751
  • 751
  • t-SNE
  • PF Heatmap
  • PF Overview
  • PF Index
  • PAT-PCA
  • PF Groups
  • Signal Distribution
t-SNE: Donor Groups
Responders vs. Non-Responders
Follow the Data

This demo data set shows six CD19 CAR-T cell products from three responders and three non-responders.

Key
  • Non-Responders
  • Responders
t-SNE: Donor Groups
Responders vs. Non-Responders
Follow the Data

This demo data set shows six CD19 CAR-T cell products from three responders and three non-responders.

There are clear functional differences between the responders and non-responders.

But what are those differences?

Find out in the next t-SNE view.

Key
  • Non-Responders
  • Responders
t-SNE: Polyfunctionality
Compare the Degree of Polyfunctionality
Follow the Data

We can see that the responders have the presence of highly polyfunctional CAR-T cells.

These highly polyfunctional cells have correlated to anti-tumor activity, persistence, and durability in a variety of studies.

What are the biological drivers of this increased polyfunctionality?

Find out in the next view.

Key
  • 5+
  • 4
  • 3
  • 2
  • 1
  • 0
t-SNE: Dominant Functional Group
Explore the Secreted Cytokine Profile
Follow the Data

The highly polyfunctional subsets of CAR-T cells in the responder groups are driven by a variety of cytokines.

As shown in this view, we can see that the stimulatory, effector, and chemoattractive cytokines play a dominant role.

Key
  • Inflammatory
  • Regulatory
  • Chemoattractive
  • Stimulatory
  • Effector
  • Non-secretor
Heatmap
Single-Cell Polyfunctional Heatmap
Follow the Data

Here you can see that the Responders have more polyfunctional cells than the Non-Responders.

Key
  • 5%
  • 2.5%
  • 1%
  • 0.5%
  • 0.1%
Heatmap Key
PF Overview
Single-Cell Polyfunctional Overview
Follow the Data

It is clear that the percentage of polyfuctional cells in the Responders is much higher than in the Non-Responders.

Key
  • 2 cytokines
  • 3 cytokines
  • 4 cytokines
  • 5+ cytokines
CD4 & CD8 Markers
Polyfunctional Overview
Follow the Data

It is clear that the percentage of polyfuctional cells in the Responders is much higher than in the Non-Responders

Key
  • 2 cytokines
  • 3 cytokines
  • 4 cytokines
  • 5+ cytokines
PSI
Single-Cell Polyfunctional Strength Index
Follow the Data

Responders have higher polyfunctional immune response driven by primarily stimulatory and effector cytokines

Key
  • Effector
    Granzyme B, IFN-g, MiIP-1a, Perforin, TNF-a, TNF-b
  • Stimulatory
    GM-CSF, IL12, IL-15, IL-2, IL-21, IL-5, IL-8, IL-9
  • Chemoattractive
    CCL-11, IP-10, MIP-1b, RANTES
  • Regulatory
    IL-10, IL-13, IL-22, IL-4, TGF-b1, sCD137, sCD40L
  • Inflammatory
    IL-17A, IL-17F, IL-1b, IL-6, MCP-1, MCP-4
Key
  • Effector
    Granzyme B, IFN-g, MiIP-1a, Perforin, TNF-a, TNF-b
  • Stimulatory
    GM-CSF, IL12, IL-15, IL-2, IL-21, IL-5, IL-8, IL-9
  • Chemoattractive
    CCL-11, IP-10, MIP-1b, RANTES
  • Regulatory
    IL-10, IL-13, IL-22, IL-4, TGF-b1, sCD137, sCD40L
  • Inflammatory
    IL-17A, IL-17F, IL-1b, IL-6, MCP-1, MCP-4
PSI
Single-Cell Polyfunctional Strength Index
Follow the Data

Responders have higher polyfunctional immune response driven by primarily stimulatory and effector cytokines

Key
  • Effector
    Granzyme B, IFN-g, MiIP-1a, Perforin, TNF-a, TNF-b
  • Stimulatory
    GM-CSF, IL12, IL-15, IL-2, IL-21, IL-5, IL-8, IL-9
  • Chemoattractive
    CCL-11, IP-10, MIP-1b, RANTES
  • Regulatory
    IL-10, IL-13, IL-22, IL-4, TGF-b1, sCD137, sCD40L
  • Inflammatory
    IL-17A, IL-17F, IL-1b, IL-6, MCP-1, MCP-4
Secreting vs. All
Single-Cell Polyfunctional Strength Index
Follow the Data

Responders have higher polyfunctional immune response driven by primarily stimulatory and effector cytokines

Secreting vs. All
Single-Cell Polyfunctional Strength Index
Follow the Data

The PSI metric helps you predict that the patients in Therapy 1 are going to respond better to this particular therapy than Therapy 2 patients.