Schedule a Live Demo
IsoSpeak Interactive Tour
DATA VISUALIZATIONS
Start Tour
For the optimal tour experience, please visit this page on your Windows or Mac desktop computer.
Please visit this page on your Windows or Mac Desktop computer to experience the full IsoSpeak Guided Tour. Please visit this page on your Windows or Mac Desktop computer to experience the full IsoSpeak Guided Tour. If you are using a mobile a device, you can visit to experience a mobile version of the tour.
  • Project Info
  • Data Processing
  • Data Overview
  • Data Visualization
General
  • No Cells
  • Single-Cell
  • Two-Cell
  • Clear Selected
    Select All
    Average Duplicates
Conditions
  • Unlabeled
  • CD19
  • NGFR
  • Clear
    All
    Average
Donors
  • Unlabeled
  • D0001
  • D0005
  • D0006
  • Clear
    All
    Average
Donor Groups
  • Unlabeled
  • Non-Responders
  • Responders
  • Clear
    All
    Average
Markers
  • UNLABELED
  • CD4+
  • CD8+
  • Clear
    All
    Average
Cytokines
  • Clear
  • Secreting
  • All
  • CCL-11
  • GM-CSF
  • GRANZYME
  • IFN-G
  • IL-21
  • IL-22
  • IL-4
  • IL-5
  • IL-17A
  • IL-17F
  • IL-1B
  • IL-9
  • IL-10
  • MCP-4
  • MCP-1a
  • MCP-1B
  • PETFERIN
  • RANTES
  • TGF-B1
  • TGF-B1
  • TGF-B1
  • sCD4DL
  • 456
  • 532
  • 635
  • 751
  • 751
  • t-SNE
  • PF Heatmap
  • PF Overview
  • PF Index
  • PAT-PCA
  • PF Groups
  • Signal Distribution
t-SNE: Donor Groups
Responders vs. Non-Responders
Follow the Data

This demo data set shows six CD19 CAR-T cell products from three responders and three non-responders.

Key
  • Non-Responders
  • Responders
t-SNE: Donor Groups
Responders vs. Non-Responders
Follow the Data

This demo data set shows six CD19 CAR-T cell products from three responders and three non-responders.

There are clear functional differences between the responders and non-responders.

But what are those differences?

Find out in the next t-SNE view.

Key
  • Non-Responders
  • Responders
t-SNE: Polyfunctionality
Compare the Degree of Polyfunctionality
Follow the Data

We can see that the responders have the presence of highly polyfunctional CAR-T cells.

These highly polyfunctional cells have correlated to anti-tumor activity, persistence, and durability in a variety of studies.

What are the biological drivers of this increased polyfunctionality?

Find out in the next view.

Key
  • 5+
  • 4
  • 3
  • 2
  • 1
  • 0
t-SNE: Dominant Functional Group
Explore the Secreted Cytokine Profile
Follow the Data

The highly polyfunctional subsets of CAR-T cells in the responder groups are driven by a variety of cytokines.

As shown in this view, we can see that the stimulatory, effector, and chemoattractive cytokines play a dominant role.

Key
  • Inflammatory
  • Regulatory
  • Chemoattractive
  • Stimulatory
  • Effector
  • Non-secretor
Heatmap
Single-Cell Polyfunctional Heatmap
Follow the Data

Here you can see that the Responders have more polyfunctional cells than the Non-Responders.

Key
  • 5%
  • 2.5%
  • 1%
  • 0.5%
  • 0.1%
Heatmap Key
PF Overview
Single-Cell Polyfunctional Overview
Follow the Data

It is clear that the percentage of polyfuctional cells in the Responders is much higher than in the Non-Responders.

Key
  • 2 cytokines
  • 3 cytokines
  • 4 cytokines
  • 5+ cytokines
CD4 & CD8 Markers
Polyfunctional Overview
Follow the Data

It is clear that the percentage of polyfuctional cells in the Responders is much higher than in the Non-Responders

Key
  • 2 cytokines
  • 3 cytokines
  • 4 cytokines
  • 5+ cytokines
PSI
Single-Cell Polyfunctional Strength Index
Follow the Data

Responders have higher polyfunctional immune response driven by primarily stimulatory and effector cytokines

Key
  • Effector
    Granzyme B, IFN-g, MiIP-1a, Perforin, TNF-a, TNF-b
  • Stimulatory
    GM-CSF, IL12, IL-15, IL-2, IL-21, IL-5, IL-8, IL-9
  • Chemoattractive
    CCL-11, IP-10, MIP-1b, RANTES
  • Regulatory
    IL-10, IL-13, IL-22, IL-4, TGF-b1, sCD137, sCD40L
  • Inflammatory
    IL-17A, IL-17F, IL-1b, IL-6, MCP-1, MCP-4
Key
  • Effector
    Granzyme B, IFN-g, MiIP-1a, Perforin, TNF-a, TNF-b
  • Stimulatory
    GM-CSF, IL12, IL-15, IL-2, IL-21, IL-5, IL-8, IL-9
  • Chemoattractive
    CCL-11, IP-10, MIP-1b, RANTES
  • Regulatory
    IL-10, IL-13, IL-22, IL-4, TGF-b1, sCD137, sCD40L
  • Inflammatory
    IL-17A, IL-17F, IL-1b, IL-6, MCP-1, MCP-4
PSI
Single-Cell Polyfunctional Strength Index
Follow the Data

Responders have higher polyfunctional immune response driven by primarily stimulatory and effector cytokines

Key
  • Effector
    Granzyme B, IFN-g, MiIP-1a, Perforin, TNF-a, TNF-b
  • Stimulatory
    GM-CSF, IL12, IL-15, IL-2, IL-21, IL-5, IL-8, IL-9
  • Chemoattractive
    CCL-11, IP-10, MIP-1b, RANTES
  • Regulatory
    IL-10, IL-13, IL-22, IL-4, TGF-b1, sCD137, sCD40L
  • Inflammatory
    IL-17A, IL-17F, IL-1b, IL-6, MCP-1, MCP-4
Secreting vs. All
Single-Cell Polyfunctional Strength Index
Follow the Data

Responders have higher polyfunctional immune response driven by primarily stimulatory and effector cytokines

Secreting vs. All
Single-Cell Polyfunctional Strength Index
Follow the Data

The PSI metric helps you predict that the patients in Therapy 1 are going to respond better to this particular therapy than Therapy 2 patients.