MOBILE VERSION
IsoSpeak Interactive Tour

You are currently on the mobile version of the IsoSpeak Interactive Tour. To experience the desktop version on your desktop computer, please follow this link.

MOBILE VERSION
IsoSpeak Interactive Tour

You are currently on the mobile version of the IsoSpeak Interactive Tour. To experience the desktop version on your desktop computer, please follow this link.

Welcome to the IsoSpeak Interactive Tour

IsoSpeak helps you make sense of complex data and instantly analyze your samples down to the individual cell, biomarker, and cytokine —all with a click of a button!

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Award-Winning Visualizations

Choose from an expansive visualization library to track how each immune cell is functionally communicating, and to identify polyfunctional cell subsets.

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Instantly Visualize Your Data

This guided tour showcases a simplified environment of the IsoSpeak software.

You'll explore four of the most powerful visualizations available to researchers using the IsoPlexis platform.

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Instantly Customize Your Data

Easily toggle on and off the data you wish to visualize and IsoSpeak will perform the analysis for you.

No more wasting precious time with tedious exports and data aggregation!

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Demo Case Study Data
Cellular & Regenerative Medicine

In this tour, you'll explore one of our internal case study data sets in the Cellular & Regenerative Medicine research area.

Read the short "Follow the Data" summary for each visualization type to learn more about the data you are seeing.

Identify Powerful Functional Drivers
t-SNE

The t-SNE provides 3D visualizations of single-cell functional cytokines and high-dimensional mapping of the unique polyfunctional subsets.

You can select three views: Donor GroupsPolyfunctionality & Dominant Functional Group.

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t-SNE

Identify Powerful Functional Drivers

t-SNE: Donor Groups

Donor groups cell mapping helps you to stratify data points from your samples by responders and non-responders.

In the next visualization you can dive deeper into the data and discover the functional differences between responders and non-responders.

t-SNE: Donor Groups
Responders vs. Non-Responders
Follow the Data

This demo data set shows six CD19 CAR-T cell products from three responders and three non-responders.

There are clear functional differences between the responders and non-responders.

But what are those differences?

Find out in the next t-SNE view.

Key
  • Non-Responders
  • Responders
t-SNE: Polyfunctionality

Polyfunctionality cell mapping helps you compare data points by the degree of the polyfunctionality of the samples.

The darker orange indicates a higher number of non-redundant cytokines secreted per single-cell.

t-SNE: Polyfunctionality
Compare the Degree of Polyfunctionality
Follow the Data

We can see that the responders have the presence of highly polyfunctional CAR-T cells.

These highly polyfunctional cells have correlated to anti-tumor activity, persistence, and durability in a variety of studies.

What are the biological drivers of this increased polyfunctionality?

Find out in the next view.

Key
  • 5+
  • 4
  • 3
  • 2
  • 1
  • 0
t-SNE: Dominant Functional Group

Dominant Functional Group cell mapping helps you explore a color-coded visualization of data points based on the cytokine profile being secreted.

These include inflammatory, regulatory, chemoattractive, stimulatory, and effector cytokine profiles. Non-secretors are displayed in grey.

t-SNE: Dominant Functional Group
Explore the Secreted Cytokine Profile
Follow the Data

The highly polyfunctional subsets of CAR-T cells in the responder groups are driven by a variety of cytokines.

As shown in this view, we can see that the stimulatory, effector, and chemoattractive cytokines play a dominant role.

Key
  • Inflammatory
  • Regulatory
  • Chemoattractive
  • Stimulatory
  • Effector
  • Non-secretor
Polyfunctional Heatmap
Heatmap

Use Polyfunctional Heatmaps to uncover the critical cell subpopulations that exist only in the condition/group of interest.

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Heatmap

Polyfunctional Heatmap

Polyfunctional Heatmap

Compare the frequency at which various functional and polyfunctional groups are secreted in your samples.

Select your desired condition/group of interest to automatically uncover the critical cell subpopulations.

Heatmap
Single-Cell Polyfunctional Heatmap
Follow the Data

Here you can see that the Responders have more polyfunctional cells than the Non-Responders.

Key
  • 5%
  • 2.5%
  • 1%
  • 0.5%
  • 0.1%
Polyfunctional Heatmap

The higher yellow density at the top displays the responders, showing that there are more polyfunctional subset of cells.

Each row corresponds to one sample and each column corresponds to a specific single cytokine or polyfunctional group.

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Polyfunctional Heatmap

The dots in each column indicate which cytokines are secreting.

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Single-Cell Polyfunctional Overview
PF Overview

This visualization reveals the polyfunctionality of your selected samples, calculated as the percentage of single cells secreting two or more cytokines.

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PF Overview

Single-Cell Polyfunctional Overview

Polyfunctional Overview

Here you can see the huge polyfunctional differences between the Non-Responders and the Responders groups.

PF Overview
Single-Cell Polyfunctional Overview
Follow the Data

It is clear that the percentage of polyfuctional cells in the Responders is much higher than in the Non-Responders.

Key
  • 2 cytokines
  • 3 cytokines
  • 4 cytokines
  • 5+ cytokines
Drill Down to Your Desired Biomarker

IsoSpeak helps you automatically filter and group your data by Conditions, Donors, Donor Groups, Biomarkers, and Cytokines.

Example: You can select the CD4+ & CD8+ biomarkers to filter this sample data set.

CD4 & CD8 Markers
Polyfunctional Overview
Follow the Data

It is clear that the percentage of polyfuctional cells in the Responders is much higher than in the Non-Responders

Key
  • 2 cytokines
  • 3 cytokines
  • 4 cytokines
  • 5+ cytokines

CD4+ & CD8+ Biomarkers: Toggle On

CD4+: Toggle On

CD8+: Toggle On

Single-Cell Polyfunctional Strength Index (PSI)
PF Index

PSI is the most novel and revealing metric for measuring the potency of different immune cell types.

It can help you accelerate your immunotherapy programs from discovery to predicting response.

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PSI
PSI

The Single-Cell Polyfunctional Strength Index (PSI) aggregates all single-cell, multi-dimensional secretions from a sample into a single index.

You can even instantly average your data without any manual exports or calculations!

PSI
Single-Cell Polyfunctional Strength Index
Follow the Data

Responders have higher polyfunctional immune response driven by primarily stimulatory and effector cytokines

Averaging Toggle On

Key
  • Effector
    Granzyme B, IFN-g, MiIP-1a, Perforin, TNF-a, TNF-b
  • Stimulatory
    GM-CSF, IL12, IL-15, IL-2, IL-21, IL-5, IL-8, IL-9
  • Chemoattractive
    CCL-11, IP-10, MIP-1b, RANTES
  • Regulatory
    IL-10, IL-13, IL-22, IL-4, TGF-b1, sCD137, sCD40L
  • Inflammatory
    IL-17A, IL-17F, IL-1b, IL-6, MCP-1, MCP-4

Averaging Toggle On

Key
  • Effector
    Granzyme B, IFN-g, MiIP-1a, Perforin, TNF-a, TNF-b
  • Stimulatory
    GM-CSF, IL12, IL-15, IL-2, IL-21, IL-5, IL-8, IL-9
  • Chemoattractive
    CCL-11, IP-10, MIP-1b, RANTES
  • Regulatory
    IL-10, IL-13, IL-22, IL-4, TGF-b1, sCD137, sCD40L
  • Inflammatory
    IL-17A, IL-17F, IL-1b, IL-6, MCP-1, MCP-4
TOUR COMPLETE
You did it!

You've completed the IsoSpeak Interactive Tour and learned about the powerful methods to visualize true functional biology. But, this is just the tip of the iceberg...