IsoPlexis News

2020

2020

Accelerating Clinical Biomarker Discovery for Curative Medicines

Clinical biomarkers are critical for the acceleration of curative medicines. The identification of these novel clinical biomarkers allows researchers to better understand complex mechanisms of immune response to diseases, facilitating the development of more effective therapeutics. Functional biomarkers can be used to predict response, identify potential effective treatments, and monitor a patient’s response to treatment. Functional biomarker discovery is leading the growth of personalized medicine, allowing researchers and clinicians to tailor treatment to a patient’s individual immune profile. IsoPlexis has the ability to detect rare cell subset with superpowers (highly functional cells) which have been proven to be highly predictive biomarkers in studies across many different research areas. This uniquely predictive functional biomarker is guiding the development of effective therapeutics for cancers, inflammation, and infectious diseases, all on an automated platform that harnesses the most powerful cells to personalize immune medicine.
2020

IsoPlexis Targets New Market Segments With Cheaper, More Flexible Single-Cell Proteomic Systems

Single-cell proteomics firm IsoPlexis is looking to expand its market reach with the launch of a lower-priced, smaller-footprint platform and new assay capabilities. The company also continues to move its technology into the clinic, having identified a blood-based biomarker of response to immunotherapy in melanoma that researchers presented on at the Society for Immunotherapy of Cancer (SITC) annual meeting this month. The IsoSpark has an 18-inch footprint and can run four chips at a time compared to eight chips simultaneously for the IsoLight system. Mackay added that the platform is targeted to the academic and biotech markets, which he said “prefer having more modular, personalized lab systems.” IsoPlexis also launched this month the IsoSpark Duo, which is more targeted to the IsoLight user base, offering the same level of throughput but increased flexibility in that it can run two different sets of chips simultaneously, allowing researchers to profile different cell types in the same experiment.
2020

IsoSpark, IsoLight, and IsoSpark Duo: Accelerating the Next Big Breakthrough in Immune Medicine

Researchers urgently need new approaches for functional immune profiling in order to accelerate discovery and development of curative medicines. The newest innovations from IsoPlexis make functional proteomics widely accessible to virtually every laboratory, speeding development of personalized, curative medicines. Now the analytic prowess IsoPlexis is known for is available in three different formats, all fully automated: the IsoSpark, IsoLight, and IsoSpark Duo. These systems detect the rare subsets of highly functional superpowered cells through measuring true function of the full range of cytokines, chemokines, and other proteins. All three systems leverage the same uniquely innovative applications in functional immune profiling, intracellular signaling omics, and high-plex automated immunoassays, accelerating therapeutic development.
2020

Harnessing the Most Powerful Cells Crucial to Accelerating Curative Medicines

IsoPlexis’ functional proteomics is changing how the world thinks about immune cell characterization, showing how harnessing the most powerful cells is changing immune medicine. The IsoSpark, a personalized proteomics system for any laboratory, makes unique functional proteomics accessible to every lab, providing an integrated and flexible solution for accelerating curative medicines. The IsoSpark, the IsoLight, and the IsoSpark Duo, along with functional immune landscaping, intracellular signaling omics, and high-plex automated immunoassays are the latest innovations from IsoPlexis. The IsoSpark, introduced on November 11th, is currently available for pre-order in North America and Europe with shipping planned for January 2021.
2020

COVID-19 Multiomic Immune Response Study Reveals Differences in Disease Progression, Severity

A team led by researchers at the Institute for Systems Biology has completed a large multiomic study characterizing the immune response in COVID-19.
2020

IsoPlexis Partnering With Yale on Assays for Assessing Immune Response to COVID-19

Single-cell proteomics firm IsoPlexis said Thursday that it is partnering with Yale University to develop assays for COVID-19 using the company’s IsoLight system. According to the company, the partners aim to identify markers of patient immune response to the virus that could be used to inform development of therapies and vaccines for the disease.
2020

The Power of Multi-Omics at the Single-Cell Level

Single-cell analysis is a relatively new technology that has revolutionized the ability of researchers to study variation within a population of cells (e.g. organs, tissues, and cell cultures). IsoPlexis’ single-cell proteomics, for example, has allowed researchers to identify the specific cytokines each single cell is secreting to identify highly functional subsets of cells that can accelerate the development of vaccines and therapeutics. The latest advance in the field of single-cell analysis is single-cell multi-omics—just last year, Nature Methods chose it as their Method of the Year “for its great impact in revealing cell functions, discovering relationships across ‘omes, and recording dynamic biological events.” IsoPlexis’ technology allows researchers to perform deep functional multi-omic analyses on the secretome, phosphoproteome, and metabolome at single-cell and population resolution, using very small sample sizes, on a single piece of equipment.  As sequencing technologies continue to advance, the IsoLight proteomics hub makes it possible to profile cells in multiple modalities on a single system—an advance that is making waves in cancer, infectious disease research, and beyond.
2020

Predicting and Overcoming Resistance Using IsoPlexis’ Single-Cell Intracellular Proteomic and Metabolomic Analysis Tools

Cancer cells develop resistance to targeted therapies, and their inherent functional heterogeneity makes it difficult for researchers to understand and characterize these resistance mechanisms. IsoPlexis’ single-cell proteomics is helping researchers overcome resistance to targeted inhibitors, leading to better strategies and combination therapies. In a recent Nature Communications article, James Heath’s team from the California Institute of Technology described using predictive single-cell intracellular proteomics and metabolomics to identify how a cancer cell line transitions to a final drug-resistant state via two distinct trajectories. Using IsoPlexis’ proteomic barcoding technology, the researchers characterized cellular heterogeneity within cell populations at different timepoints and quantitatively connected multiple timepoints to characterize dynamic heterogeneity on an individual cell level. Cellular state changes became prominent around day 3, as most probed analytes exhibited a sharp but transitory increase in variance. Understanding these functional adaptations allowed the team to predict and develop an effective therapeutic combination to overcome this adaptive resistance.
2020

IsoPlexis, Medquest Ink Distribution Deal for Singapore, Malaysia

IsoPlexis said on Tuesday that it has signed an exclusive agreement with Medquest to distribute IsoPlexis’ products in Singapore and Malaysia. “Our IsoLight platform provides a wide range of applications across numerous disease areas and we are excited for our systems to generate impactful data at leading pharmaceutical and life science institutions in Singapore and Malaysia,” IsoPlexis CEO Sean Mackay said in a statement. Last week, IsoPlexis said that Switzerland-based Lonza will use the IsoLight platform for functional quality analytics for cell therapy products produced on Lonza’s automated cell manufacturing platform. Also in August, IsoPlexis said it is partnering with researchers at Columbia University to study immune response in COVID-19 patients.
2020

Highly Automated Precision Medicine is the Future of Cellular Immunotherapy

Challenges remain in cell therapy manufacturing optimization and scalability, especially when developing autologous therapies. In a recent GEN webinar, Matthew Hewitt, PhDhead of R&D (a.i.) and clinical development, personalized medicine at Lonza, and Stacey Willard, PhD, director of product management at IsoPlexis, provided an overview of the Cocoon™ cell therapy manufacturing platform and the IsoLight™ single-cell proteomics system along with data from case studies. Combining the IsoLight system with the Cocoon platform allows quantification of the true functional biology of the cell product. Although other analytic approaches provide rich data, they do not reveal single cell functional detail, which facilitates better predictions of potency, durability, and adverse effects. “This collaboration between Lonza and IsoPlexis highlights the evolution of cell therapy manufacturing. As we continue to scale the manufacturing of cell therapies, it will be critical to characterize the quality and performance of these products with technologies such as IsoPlexis’ IsoLight,” said Hewitt.
2020

IsoPlexis Names PhenoVista Biosciences A Certified Service Provider

PhenoVista Biosciences, a California-based company with an unmatched combination of imaging expertise, assay development experience, and the latest technologies and data analysis capabilities, was selected as a Certified Service Provider of IsoPlexis’ products. PhenoVista can now offer the full array of IsoPlexis’ IsoCode and CodePlex assays, single-cell and ultra-small sample volume solutions which accelerate workflows and are of critical use in the study of cancer immunology, infectious diseases, inflammation, vaccine development, and cell and gene therapy research. “We are excited to have named PhenoVista a Certified Service Provider for the IsoPlexis platform,” said Sean Mackay, Chief Executive Officer of IsoPlexis. “We look forward to our unique single-cell proteomics platform being widely accessible through PhenoVista, enabling its use in leading research institutions at the forefront of scientific discovery.”
2020

IsoPlexis and Lonza Collaborate on Cell Therapy Manufacturing

IsoPlexis, which markets single-cell functional proteomics products, is collaborating with Lonza, a CDMO, to use IsoPlexis’ IsoLight platform for cell and gene therapy manufacturing. The IsoLight will provide quality analytics for cell therapy products generated on Lonza’s Cocoon, an automated, highly flexible cell therapy manufacturing platform, according to Sean Mackay, CEO of IsoPlexis. The Cocoon allows for centralized and decentralized manufacturing of novel cell therapies, and the IsoLight provides a solution for the functional characterization of the cell therapy product, to ensure correlative functional potency of the manufactured cell product, continues Mackay.
“This collaboration between Lonza and IsoPlexis highlights the evolution of cell therapy manufacturing,” notes Matthew Hewitt, PhD, Head of Clinical Development and Personalized Medicine at Lonza. “As we continue to scale the manufacturing of cell therapies, it will be critical to characterize the quality and performance of these products with technologies such as IsoPlexis’ IsoLight.”
2020

IsoPlexis Partnering With Columbia to Study Immune Response to SARS-CoV-2

Single-cell proteomics firm IsoPlexis said today that it is partnering with researchers at the Department of Medicine at Columbia University to study the immune response in COVID-19 patients. The Branford, Connecticut-based company said it plans to use its single-cell proteomics platform to identify drivers of durable and protective immune responses to the virus, with the ultimate goal of using that information to inform therapeutics development.
2020

Nature Biotechnology News Feature: A singular view of COVID-19

Single-cell analysis sheds light on immune response to COVID-19 infection, enables the rapid discovery of antibody leads, and points to ways to get ahead of future pandemics. Proteomic analysis can also fill in critical gaps concerning cell type and function. A Seattle-based research consortium headed by the Swedish Medical Center, the Institute for Systems Biology and Merck is also applying a host of multi-omic analytical techniques to blood specimens collected from patients with COVID-19, including a platform developed by IsoPlexis that employs microchip-based immunoassays to profile secreted cytokines and other signaling molecules from individual immune cells. “It basically allows you to perform 35-plex analysis of cytokine secretion from viable single cells,” says James Heath, president of the Institute for Systems Biology and cofounder of IsoPlexis.
2020

FlowMetric Life Sciences, Inc. selected as IsoPlexis Certified Service Provider

FlowMetric Life Sciences, Inc., a global leader in Flow Cytometry services, and  IsoPlexis, the leader in functional single-cell proteomics, announce FlowMetric as a Certified Service Provider of the IsoPlexis technology. This certification was awarded after FlowMetric met the rigorous training and testing requirements set out by IsoPlexis.  FlowMetric can now provide Single-Cell Functional Proteomic services to its Pharmaceutical and Biotechnology customers to complement its decade-long expertise in developing and validating multi-parameter Flow Cytometry-based approaches for drug development.  FlowMetric is the only Contract Research Organization (CRO) on the East Coast to offer the menu of IsoPlexis’ IsoCode & CodePlex assays for the study of cancer immunology, inflammation, vaccine development, and cell and gene therapy research.  The IsoPlexis technology provides comprehensive workflows that combine proprietary content and bioinformatic solutions to enable large data sets to be acquired from small sample volumes.
2020

Insider’s Guide Marketplace: IsoLight (IsoPlexis)

Automate your entire workflow with one system. The IsoLight is a hub for comprehensive functional profiling of each cell type across a large assay menu of single-cell chip and software products, such as the newly released CodePlex Secretome solution, which enables quick and cost-efficient population assays and can selectively run 8 samples per chip in “MacroChambers” across 8 chips on a single run. Run an entire multiplexed ELISA workflow in a completely automated and hands-off manner. Just add your sample and walk away, achieving fully analyzed data on the same day. No additional equipment is needed. That means no washing and incubation stations, no centrifuge, no vortexer, and no plate reader. All of your proteomic needs are integrated into the completely automated IsoLight system.
2020

Fighting COVID-19 with Single-Cell Proteomics Technologies

For years, the relative abundance of particular proteins has been estimated from mRNAs in bulk tissue samples. Single-cell transcriptomics allowed a more pinpointed inference of protein levels based on detected RNA transcripts. But now the nascent field of single-cell proteomics promises direct measurements of the proteins within or secreted from individual cells. Here is a look at the emerging field of single-cell proteomics and recent applications of its technologies to combating SARS-CoV-2, the virus responsible for the COVID-19 pandemic. IsoPlexis offers the IsoLight platform for highly multiplexed single-cell proteomics of over 30 cytokines released from individual cells. Known as functional phenotyping, the results of IsoPlexis’s Single-Cell Secretome solution allow researchers to take proteomic snapshots of individual immune cells by measuring the many cytokines they secrete. Applications of their technology are helping to predict which patients might respond to particular therapies for blood and solid tumor cancers as well as infectious disease, says Sean Mackay, co-founder and CEO of IsoPlexis. “Our technology allows clinical researchers to uniquely pinpoint each single cell’s functional phenotype that influences the course of therapy, and identifying these cell subsets has helped predict durable and quality immune response correlating to outcome preclinically and in the clinic,” he says.
2020

IsoPlexis Receives $2M SBIR Grant for Alzheimer’s Research

Single-cell proteomics firm IsoPlexis said Tuesday that it has received a $2 million Small Business Innovation Research (SBIR) grant from the National Institute on Aging of the National Institutes of Health. The Phase 2 grant is for work the company is doing to analyze the proteomic responses of trafficking leukocytes in patients with Alzheimer’s disease and related neurodegenerative conditions. IsoPlexis is developing assays on its IsoLight system for detecting the inflammatory immune response generated by cells including peripheral monocytes, highly polyfunctional secretomic T cells, and microglia and exploring whether these profiles could be used for diagnosing and monitoring inflammatory neurodegenerative diseases.
2020

Single-Cell Proteomics Tackles Vaccine Development

Vaccine development can be daunting. The overarching goal to create long-lasting immune protection requires an in-depth understanding of the immune system’s response to an infectious agent, immune monitoring to determine and predict the building of the protective response, as well as cytokine-level monitoring for potential toxicities related to cytokine storms. GEN spoke to five leading researchers to hear their views and strategies on infectious disease research and vaccine development.
2020

Merck joins Seattle consortium to uncover COVID-19’s molecular workings

Merck & Co. has begun working with the non-profit Institute for Systems Biology and a consortium of companies to investigate the molecular mechanisms behind the novel coronavirus, in a bid to identify new targets for medicines and vaccines. Late last month, the ISB launched a study with health workers from Swedish Medical Center, also based in the institute’s hometown of Seattle, to explore why certain COVID-19 patients die or require intensive care while others may show no symptoms at all. Both the ISB and Swedish are part of the Providence St. Joseph Health network.

Other participants in the study include health workers within Providence St. Joseph Health and the Swedish Medical System, as well as collaborators from Stanford University, IsoPlexis, Adaptive Biotechnologies, 10x Genomics, Metabolon and others.

Merck will provide research funding and work with ISB researchers to find targets for potential interventions, including drugs, antibody therapies and vaccines. The study will initially analyze samples from 200 patients with the potential to expand to 300.

2020

Proteomics Firms Redirect Immuno-oncology Tools to COVID-19 Research

In recent years, developments in single cell and single molecule techniques have allowed proteomics researchers to characterize the body’s immune response with improved depth and resolution. Much of this research has been focused on immuno-oncology, but as the SARS-CoV-2 outbreak has turned into a global pandemic, a number of companies and researchers in this space have turned their attention to better understanding how patients’ immune systems respond to the virus. Single-cell proteomics firm IsoPlexis has to date largely marketed its IsoLight platform to researchers doing work on cancer immunotherapy, where it has proved useful for assessing immune cell cytokine production. Given the apparently important role of overactive cytokine production, the so-called cytokine storm, in COVID-19 deaths, the company is now working with researchers at the Institute for Systems Biology in Seattle to analyze the cytokine production of immune cells taken from COVID-19 patients.
2020

IsoPlexis, ISB Partner on COVID-19 Single-Cell Functional Immune Response Mapping

Functional cellular proteomics platform developer IsoPlexis and the Institute for Systems Biology (ISB) said today they will partner to study COVID-19 by mapping functional immune responses at the single cell level. The partners said they plan to carry out research on immune cells from people who have been diagnosed with, or recovered from, COVID-19. IsoPlexis’ single cell functional proteomics platform will be applied on a variety of immune cell types, including various T cells and myeloid cells, in order to map the overall response to the virus. According to IsoPlexis, uncovering functional immune responses using its technologies has led to key breakthroughs in therapies that harness the immune system across disease areas. “Global effort and partnerships are needed to reach an accelerated understanding of COVID-19,” added ISB president and professor James R. Heath, PhD. Speaking with GEN last year, Heath discussed how single-cell functional analyses accelerated development of CAR-T therapies. The analysis of CAR-T cells prior to infusion, he noted, can help researchers anticipate patient response, which also provides feedback usable in the process of manufacturing cells; “You want to control the manufacturing process to optimize the tumor killing properties of the T-cells, while also minimizing factors such as T cell exhaustion. “These single-cell functional analyses have been pretty good across the board at differentiating responders from non-responders, both during the course of therapy and, in some published cases, in terms of analyzing CAR-T-cell products before they are even infused into the patient,” Heath observed. “There are not many other metrics that have yet emerged that give you that type of feedback.”
2020

Proteomics Labs Exploring COVID-19 Therapeutic Options, Dx Development

Single-cell proteomics firm IsoPlexis is collaborating with researchers at the Institute for Systems Biology in Seattle to look at the immune response in patients who have been diagnosed or recovered from COVID-19, with the goal of better understanding what factors are involved in better and worse outcomes. The company’s IsoLight platform is capable of measuring dozens of proteins in single cells and has found a niche within cancer immunotherapy where researchers are using it to look at immune cell cytokine production. The company plans to similarly analyze the cytokine production profiles of immune cells taken from COVID-19 patients, said Sean Mackay, the company’s co-founder and CEO. IsoPlexis also plans to work with the ISB to make bulk measurements of cytokines secreted by patient immune cells, with the goal of better understanding which people may be more susceptible to the “cytokine storm” phenomenon where an overactive immune response causes severe illness or death in certain patients.
2020

IsoPlexis, Institute for Systems Biology Partner to Identify COVID-19 Immune Mechanisms

IsoPlexis has partnered with the Institute for Systems Biology (ISB) to map functional immune response to COVID-19 at the single-cell level. The group will use IsoPlexis’ single-cell functional platform to analyze immune cells from individuals who have been diagnosed or recovered from COVID-19. The team aims to examine a variety of immune cell types—including various T cells and myeloid cells—to put together a map of the overall response. “Through our unique functional analysis of each cell, we can unlock further understanding of how COVID-19 interacts with the immune system,” Sean Mackay, CEO and cofounder of IsoPlexis, said in a statement. “Cellular immune signatures based on cytokines may be key in understanding and predicting response, and also how to mitigate disease progression.”
2020

IsoPlexis CodePlex Secretome and Single-Cell Innate & Myeloid Solution

IsoPlexis has released two new products for use with its IsoLight cellular proteomics instrument. CodePlex Secretome is a fully automated approach to obtaining highly multiplexed bulk cytokine data that requires five minutes of hands-on time for sample loading. CodePlex Secretome multiplexes more than 30 cytokines, and is modular so users can simultaneously examine as few as eight samples or as many as 64. The new Single-Cell Innate & Myeloid solution detects the functional single-cell differences from innate and myeloid cells by identifying what each cell secretes in a highly multiplexed (32-plus cytokines) manner to reveal the sources of cellular difference through functional phenotyping. It joins other IsoLight single-cell chip applications which allow researchers to visualize single-cell-level data and pinpoint biological drivers.
2020

IsoPlexis Enables Discovery of New Cell Types to Accelerate Cancer Immunology

Unable to be detected by conventional technologies, cellular functional heterogeneity leads to differences in patient response and disease progression. Filling this gap, IsoPlexis’ single-cell functional proteomics is allowing researchers to characterize the full range of extracellular functions (32+ cytokines) per single cell. By exploring, understanding, and characterizing cells based on function, researchers can accelerate therapeutic development by revealing new phenotypes that specifically correlate to individual disease or response states.
2020

IsoPlexis Demonstrates Expanded Cell Profiling Capabilities in Immunotherapy Study

In a recent study led by researchers at the University of California, Los Angeles, proteomics firm IsoPlexis demonstrated the capabilities of its expanded single-cell analysis platform. In the work, which was detailed in a paper published last month in Nature Communications, scientists used the company’s platform to profile the cytokines produced by both T cells and natural killer (NK) cells in the blood of melanoma patients receiving adoptive cell transfer (ACT) therapy. IsoPlexis is currently expanding its offerings to target a variety of different immune cell types, said Sean Mackay, the company’s co-founder and CEO. From IsoPlexis’ perspective, the analysis of NK cells is significant in that it demonstrates the ability of its platform to look beyond T cells and to effectively analyze NK cells circulating in patient blood. “This is the first time that has been published,” Mackay said, adding that it marks an important step for the company’s efforts to move into cancer immunology more broadly. The NK cell analysis used the same cytokine chip that IsoPlexis has offered for T cell research. However, Mackay said the company is developing new cytokine chips specific for macrophages and monocytes that will better address those cells’ specific cytokine repertoires. The company is also releasing this quarter a panel aimed at bulk cell analysis of cytokines. While IsoPlexis has focused primarily on single-cell analysis, Mackay said it has received requests from customers interested in doing comparisons of single-cell and bulk cytokine profiles.
2020

Nektar Therapeutics Announces Publication of Two Manuscripts on Lead Immuno-oncology Candidate, Bempegaldesleukin (Bempeg) in Nature Communications

Nektar Therapeutics today announced the publication of preclinical data on its lead immuno-oncology candidate, NKTR-214, bempegaldesleukin (bempeg) in two manuscripts in Nature Communications. Bempeg is an investigational CD122-preferential interleukin-2 (IL-2) pathway agonist designed to provide activation and proliferation of cancer-killing immune cells, known as CD8+ effector T cells and natural killer (NK) cells. The published data demonstrate that bempeg, in combination with immune-based therapies including checkpoint inhibition (CPI), antigen-specific vaccination and adoptive cell transfer (ACT) therapy, enhanced T-cell mediated tumor control by selectively expanding effector T cells (Teffs) over T regulatory cells (Tregs) in the tumor microenvironment. In the second manuscript, published this week in Nature Communications, a research team led by Antoni Ribas, Ph.D. at the UCLA Jonsson Comprehensive Cancer Center evaluated bempeg in combination with adoptively transferred T cell therapy (ACT) in melanoma models. The studies found ACT therapy supported by bempeg increases the proliferation, homing and persistence of anti-tumor T cells compared to ACT with IL-2, resulting in superior antitumor activity in the B16 melanoma model.
2020

Immuno-oncology combination for melanoma using Nektar drug boosts cell therapy in mice

Therapies that entail removing immune cells from patients and engineering them to recognize and fight cancer have revolutionized the treatment of some blood cancers, but they’ve proven difficult to translate to other tumor types. Researchers at the University of California, Los Angeles (UCLA) Jonsson Comprehensive Cancer Center believe they’ve hit upon a combination treatment that could help expand the use of cell therapy. The UCLA team is planning future studies to better understand how signaling pathways contribute to the anti-cancer effects they observed in mice given the T-cell combination therapy, they wrote in the study. The synergy they’ve seen so far, they said, “suggests that in patients with cancer, NKTR-214-containing regimens could increase tumor control without exacerbating systemic inflammation.
2020

Designing Next-Generation Regulatory T Cell Therapies with Single-Cell Proteomics

In a January 2020 Genetic Engineering & Biotechnology News webinar, Leonardo Ferreira, PhD, Postdoctoral Scholar and Jeffrey G. Klein Family Diabetes Fellow at the University of California, San Francisco, and Jonathan Chen, technology co-inventor at IsoPlexis, discussed CAR Tregs and how the IsoPlexis IsoLight single-cell functional proteomics system is crucial in the informed design and development of this next generation of cell therapies. To view the on demand webinar, visit here.
2020

The Decade In Proteomics: Most Significant New Applications

The last decade was an eventful one for proteomics as the field saw a number of advances ranging from new workflows and applications to improvements in instrumentation and bioinformatic innovations. Immunoassay-based methods aimed at measuring relatively large numbers of proteins at the single-cell level also saw significant advances during the decade. This was particularly true on the commercial side, with companies including Fluidigm, NanoString Technologies, IsoPlexis, Akoya Biosciences, Zellkraftwerk, IonPath, and Mission Bio launching tools for single-cell protein analysis during the last decade.
2020

In 2019, Gains in Throughput Position Proteomics for Future Clinical Impact

After two-plus decades spent struggling to drive experiment run times down to more manageable levels, a sense emerged this year within the field that this challenge had at last been met. Single-cell proteomic technologies also saw continued growth with a number of new developments in what is becoming an increasingly crowded field. Branford, Connecticut-based IsoPlexis closed a $25 million Series C round that it is using to launch several new single-cell protein assays as well as expand its operations across the US, Europe, and Asia.
2020

IsoPlexis’ $20M Financing to Enable Continued Commercial Expansion of its Single-Cell Platforms

Single-cell functional proteomics platform developer IsoPlexis announced this week it has raised an additional $20 million in a Series C round, capital that will enable the company to continue the global expansion of its flagship IsoLight single-cell proteomic analysis platform. According to the company, its technology has been used in a number of different settings including precision drug discovery and biomarker discovery in oncology, to identify proteomic differences that are often undetectable via other methods.
2020

IsoPlexis Raises Additional $20M as Part of Series C Round

IsoPlexis said today that it has raised an additional $20 million as part of its Series C financing. That follows the $25 million the company announced in May 2019, bringing the total figure for the round to $45 million. The company said it plans to use the funds to drive commercialization of its IsoLight single-cell proteomic system. IsoPlexis has 130 employees and recently expanded its presence throughout the US, Europe, and Asia.
2020

GEN Interview with Sean Mackay

GEN interviews CEO and Co-founder of IsoPlexis, Sean Mackay, who discusses some of the biggest advancements in the field, how IsoPlexis is supporting the industry, upcoming new products, and the company’s vision for the next 5-10 years.

2019

2019

IsoPlexis Wins $2M SBIR Grant From NCI

Single-cell proteomics firm IsoPlexis said today it has received a $2 million grant from the National Cancer Institute’s Small Business Innovation Research (SBIR) Development Center. The grant will fund development of methods using the company’s IsoLight system to study protein signaling pathways in single tumor cells, allowing for improved analysis of cancer signaling and tumor heterogeneity.
2019

How single cell proteomics data can drive CAR-Treg-based therapies—an interview with Leonardo Ferreira

Leonardo Ferreira, a postdoctoral fellow at the University of California, San Francisco, discusses the application of single cell proteomics data to understanding the function and therapeutic potential of genetically engineered regulatory T cells.
2019

Understanding T-cell polyfunctionality: How single cell proteomics data drive CAR-T cell therapy research and development

Vladimir Senyukov, Director of BioAnalytical Development at Precision Biosciences, talks about investigating T-cell cytokine production using single cell proteomics in order to unlock the therapeutic potential of allogeneic CAR-T cells.
2019

IsoPlexis’ New Software Designed for Better Insights on Single-Cell Immune Activation

IsoPlexis reported today that it has launched IsoSpeak software for automated on-site analysis and advanced, functional, single-cell mapping. “People see well-published data showing that proteomics can confirm how each sample is performing. This information is critical for developing immunotherapy applications. Until today, most technologies for gathering this data involved much complexity and great difficulty,” explains Mackay. “There are thousands of data points per sample which, in theory, require a lot of informatics and computers to put the data together. Our software takes all that information and those multi-data points and condenses it in your hand in an easy-to-use fashion. Getting the data is quick, and once the run is done, all those data visualizations are able to be manipulated by users on the same day.”
2019

IsoPlexis’ New Software & Applications Pave the Way for Novel Functional Understanding of Single-Cell Immune Activation and Further Application Expansion

IsoPlexis also expanded its applications with new IsoCode Chip products, including the murine cell Single Cell Polyfunctional Strength solution to open up a variety of previously published insights in the pre-clinical realm. Additionally, the Single Cell Polyfunctional Inflammation solution helps discover and target important inflammatory cell types, in high need areas like auto-immune disease, neuro-inflammation, and immune related adverse events.
2019

Improving CAR-T Cell Precision Bioprocessing with Functional Proteomics

We explore how automated cell-manufacturing platforms, such as the Miltenyi Biotec CliniMACS Prodigy®, and single-cell technology from IsoPlexis can be used to produce and assess the functional biology of next generation cell therapy products. With a deeper understanding of cellular function by characterizing the functional cytokines from each single cell, scientists can analyze how the CAR-T cells are likely to impact the immune system, which can translate to how the therapy would affect patient outcome.
2019

Polyfunctionality Correlates with In Vivo Response to Immune Checkpoint Inhibitor Therapies

Studies indicate IsoPlexis’ Polyfunctional Strength Index (PSI) has the potential to be a clinically relevant biomarker of early immune checkpoint inhibitor response
2019

IsoPlexis and Miltenyi Report on Synergistic CAR-T Cell Technologies

IsoPlexis, which recently received an NCI grant, reports that it has published a technology note with Miltenyi Biotec in a co-marketing agreement.
2019

IsoPlexis Inks Distribution Deal With BioStream for Japan

IsoPlexis said today that it has entered an exclusive agreement with BioStream to distribute its single-cell protein analysis products in Japan.
2019

Optimizing Immunotherapy Lead Selection

An urgent need exists for novel detection strategies to better understand mechanistic function and efficacy at the single-cell level in a way that correlates to clinical outcomes. Population level studies do not provide the necessary insight into immune response heterogeneity at the single-cell level. IsoPlexis’ single-cell proteomics systems address this challenge by connecting each immune cell to the many cytokines they secrete, which orchestrate the immune system.
2019

Quality and Polyfunctionality in Cell Therapy Development Can Broaden Therapeutic Options

There are no currently well-defined methods to predict or characterize adverse effects, such as severe cytokine release syndrome (CRS) or neurotoxicity. Although circulating cytokine levels in blood are monitored, often that is too late, and drugs, such as IL-6, cannot prevent the patient’s death. In addition, the CAR T-cell infusion product may contain some cells with bizarre functions. IsoPlexis’ technology can comprehensively evaluate the cells with almost no bias, so you get a full portrait of the whole infusion product, even small subsets of the cells with bizarre functions.
2019

Signs, Portents, and Immuno-Oncology Biomarkers

Single-cell suspensions don’t present spatial context like needle biopsies do, but the cytokines each individual cell produces still tell a compelling story. For example, cytokine secretion profiles correlate with, and can help predict, clinical outcomes in immunotherapy, said Will Singleterry, PhD, director of business development at IsoPlexis. To back this assertion, he presented data from the company’s many collaborations.
2019

The Power of PSI in Evaluating CAR-T Therapy

Researchers and clinicians urgently need new approaches to understand the functional profile of cell therapy products to accelerate discovery and development, to achieve manufacturing consistency, and to predict which patients will derive the most value from these drugs while minimizing side-effects. IsoPlexis’ Polyfunctional Strength Index (PSI™) can be utilized to address these challenges, in particular to provide metrics that uniquely relate the CAR-T cell product response to in vivo preclinical and clinical outcome measures.
2019

The Polyfunctional Strength Index: From Concept to Solution

Unlike a small molecule or antibody that always has the same structure, each time an autologous cell therapy is developed the starting material comes from a different patient, making it virtually impossible to reproducibly develop an identical “drug”. Having the ability to drive better outcomes by profiling the complete function of each cell would be a significant advantage for bioengineers—a challenge the IsoPlexis single-cell proteomic system has been designed to address.
2019

Q&A: Evolution of CAR-T Cell Therapy and Future Directions

James Heath, PhD., is president and professor at the Institute for Systems Biology in Seattle. He also has the position of Professor of Molecular and Medical Pharmacology at UCLA, and he has directed the NCI-funded NSB Cancer Center since 2005. GEN recently spoke with him on current issues and future trends regarding CAR T-cell therapeutics.
2019

Improving Preclinical Discovery of CRISPR Engineered Immune Cell Therapies

There is an urgent need to characterize the potency and efficacy of CRISPR-Cas9-modified inducible pluripotent stem cell-derived natural killer cells for preclinical cancer immunotherapy research. IsoPlexis’ single-cell proteomics system addresses this challenge by connecting each immune cell to cytokine secretion and thereby correlating them to in vivo outcome across a range of disease areas.
2019

MD Anderson, IsoPlexis Link T-Cell Functionality to Immunotherapy Response in AML

In work presented at the recent American Association for Cancer Research annual meeting, they found that T-cell cytokine production predicted patient response.
2019

IsoPlexis Closes $25M Series C Round

“We are pleased to lead the financing of IsoPlexis, an emerging leader in single cell analysis, which is catalyzing research in immunotherapy as well as a number of other high value applications,” Chris MacGriff of Northpond Ventures said in a statement.
2019

IsoPlexis Wins $4M SBIR Grant From NCI

The Branford, Connecticut-based company said that it will use the funds to commercialize its IsoLight platform for use in quality control and guidance of CAR T therapy.

2018

2018

CAR T cells find strength in polyfunction

In this issue of BloodRossi et al report that the heterogeneity of personalized chimeric antigen receptor (CAR) T cells has limited the ability to identify product attributes that enhance patient outcomes, but they have showed that by using single-cell analyses, heterogeneity may actually improve clinical outcomes. Single-cell polyfunctional analyses provide a measure of product efficacy that can be used to identify patients likely to respond to treatment and those likely to develop toxicities.
2018

IsoPlexis Takes on Cancer, One Cell at a Time

As IsoPlexis prepares to take its production to the next level in 2018, two science news publications have honored the company for technology that tailors optimum treatments for cancer patients. The Scientist magazine named the company’s IsoCode chip and IsoLight platform, an all-in-one system that reads the individual cells of tumors, as the Top Innovation of 2017. One week later, FierceBiotech named the same system the 2017 Fierce Innovation Award for Technology Innovation.
2018

IsoPlexis Uses Single-Cell Platform to Assess T Cell Activity in CAR-T Cancer Therapy

The work was part of a larger study looking at the effectiveness of ACT in combination with the cytokine agonist NKTR-214. The study, led by Antoni Ribas, director of the tumor immunology program at UCLA’s Jonsson Comprehensive Cancer Center, found that the ACT-NKTR-214 combination improved the induction of cytotoxic T cells and increased their polyfunctionality, indicating that addition of NKTR-214 could boost the performance of ACT. NKTR-214 is produced by San Francisco-based biopharma company Nektar Therapeutics, which also participated in the study. “The finding in a preclinical model that the IsoPlexis assay can detect a remarkable increase in polyfunctionality in tumor-infiltrating lymphocytes recovered from tumors of mice treated with NKTR-214 compared to IL-2 is the most conclusive data we have obtained in this model system,” said Ribas.
2018

IsoPlexis has been Recognized with Pharma Tech Outlook’s Cover Story for the Top 10 Analytics Solution Providers in 2018

The award focuses on IsoPlexis’ IsoSpeak software suite’s ability to clarify results, reports, and associations from large, complex patient data sets, thereby delivering leading biopharma and clinical researchers better data by which to determine effective cancer immunotherapies.
2018

The Power of ‘Poly’ in CAR-T

Research recently published in the journal Blood — conducted in a collaborative effort with Kite Pharma, a Gilead company — demonstrates a significant association between the functionality of an anti-CD19 CAR-T cell product before treatment, as defined by IsoPlexis’ Polyfunctional Strength Index (PSI), and the objective response in patients with non-Hodgkin lymphoma (NHL). “The results highlight the potential to predict whether cancer patients will respond to CAR-T cell therapy before treatment, as well as to improve both pre-infusion product potency testing and guide cell product optimization.”

2017

2017

U.S. Senator Chris Murphy is Highlighting IsoPlexis of Branford, Connecticut, as “Murphy’s Innovator of the Month” for their Work Combating Cancer

“I’m proud to launch my series with IsoPlexis as the inaugural awardee. The folks at IsoPlexis are doing breakthrough work to make cancer treatments more effective and stop this deadly disease in its tracks—that’s something to celebrate.”
2017

Congratulations to IsoPlexis director and co-inventor Dr. James Heath, who was named President of the Institute for Systems Biology

“I am extremely honored to assume the position of president of ISB. It is also tremendously humbling to follow in Lee Hood’s footsteps. Lee is a giant of science, and he has changed the face of modern biology. Through scholarship, innovation, and an outstanding faculty, he has also built ISB into the world leader of systems biology. I can’t wait to begin working with ISB faculty to help build an ISB for the future.”
2017

Kite Announces AACR Presentation on IsoPlexis Collaboration

As described in the abstract of this clinical biomarkers presentation, the IsoCode platform uncovered that polyfunctional anti-CD19 CAR T cells determined by single-cell multiplex proteomics associated with clinical activity in patients with advanced non-Hodgkin’s lymphoma. The single-cell Polyfunctional Strength Index (PSI) of patient CAR T cells, measured on the IsoCode platform, showed a statistically significant association with objective response to CAR T therapy.
2017

IsoPlexis Research, in Collaboration with Oncology Specialist Kite Pharma, Offers Hope for Targeted CAR-T Treatment

US-based biotech firm IsoPlexis has presented findings from research using the company’s precision engineering platform, called IsoCode. The data show the potential to predict whether cancer patients will respond to CAR-T cell therapy prior to treatment, as well as to improve both pre-infusion potency testing and cell product design.
2017

CAR T Cell Functionality Found to Correlate With Outcomes for Patients With NHL

“Through this research, we were able to highlight the important role a functionally versatile subpopulation of CAR T cells may play in the fight against cancer, leading to new ways to characterize and optimize T-cell products,” Adrian Bot, MD, Kite’s vice president, Translational Medicine, said in a statement. “These insights were made possible by using the IsoPlexis technology.”
2017

Keytruda success a crowning moment for cancer immunotherapies, but are more on their way?

The licensing deals, mergers and acquisitions, and excitement at events such as ASCO in the last couple of years, have of course come in combination with the impressive data that clinical trials are yielding, notes Sean Mackay, the chief executive of IsoPlexis. Its partners include the cancer immunotherapy specialist, Kite Pharma. “The reason there is such a high potential is rooted in the data,” Mr Mackay says.
2017

IsoPlexis Using Recent Influx of Funds for Automated Single-Cell Analysis Platform

Omics firm IsoPlexis is developing an automated version of its single-cell analysis platform for use in large clinical trials of CAR-T therapies for blood cancers. The Branford, Connecticut-based company is using a $1.8 million grant it received this summer from the National Cancer Institute’s Small Business Innovation Research program to fund development of the automated system.
2017

IsoPlexis, a Branford medical technology company, developed some highly advanced hardware and software that allows oncologists to essentially read the information inside a cancer patient’s cells to see whether they would react well to immunotherapies

That means before treating a patient, doctors would be able to tell whether the immunotherapy would be effective and whether it would cause unpleasant side effects.
2017

In Branford, Finding Precision In Cancer Treatments

A Branford life sciences company is working to take the guesswork out of cancer treatments with technology that may predict how patients will respond to drugs before being exposed to them. IsoPlexis is developing a system that analyzes patients’ tumors cell by cell to determine how effective or toxic a treatment would be and to help guide doctors’ decisions about mixing different cancer therapies.
2017

The Scientist Names IsoPlexis Technology The Top Innovation of 2017

This new single-cell technology allows researchers to characterize cells based on the proteins they secrete—as many as 42 different cytokines, chemokines, and other molecule types at once. “The IsoLight single-cell technology, with its ease-of-use, has the potential to impact cancer research for both biomarker discovery and patient monitoring.”
2017

IsoPlexis Wins 2017 Fierce Technology Innovation Award

The Life Sciences Innovation Report showcases outstanding innovation that is driving improvements and transforming the industry. “Our ability to measure a lot more functional data per individual patient immune cell has provided a pathway to understanding how immunotherapy works, said Sean Mackay, Co-Founder and CEO of IsoPlexis. Mackay said there are three elements that make IsoPlexis  fierce; its thought leadership in technology that stems from the company’s scientific founders and advisory board; a very strong technology platform; and its employees. “All of those elements allow us to maintain our ferocity.”
2017

Researchers Uncover Cell Changes Behind Therapy-Resistant Cancers, Call for New Clinical Approaches

A UCLA study in collaboration with the California Institute of Technology (Caltech) shows that skin cancer cells could be chemically changed from within to reflexively alter gene expression patterns and intracellular pathways, which allows the cells to become resistant to targeted drugs. The UCLA-Caltech team conducted functional proteomics analysis of single melanoma cells, using a microfluidic single-cell barcode “lab on a chip” to determine how cellular responses varied to BRAF inhibitors.

2016

2016

NCI Alliance for Nanotechnology in Cancer: Phase II Program Summary

Researchers at this Center have applied single cell proteomics studies towards understanding patient responses to immunotherapy in clinical trials. This technology has been translated into the commercial sector for cancer immunotherapy applications by IsoPlexis, a company co-founded by former NSBCC postdoc Dr. Rong Fan (now on the faculty at Yale), and with scientific support from NSBCC Project Lead Dr. Toni Ribas.
2016

CalTech, UCSD Team Uses Single-Cell Phosphoproteomics to Predict Cancer Drug Resistance

A team led by researchers at the California Institute of Technology and the University of California, San Diego, has used single-cell phosphoproteomics to detect changes in protein signaling linked to the development of drug resistance in glioblastoma.
2016

Experimental Therapy for Brain Cancer Developed by UCLA and Caltech could Prevent Drug Resistance

The research was led by James Heath, co-director of the UCLA Jonsson Comprehensive Cancer Center’s Nanotechnology Program. Heath is the founder and a board member of IsoPlexis, a company that is seeking to commercialize the technologies used in the study.