Utilizing CodePlex Secretome for Rapid Analysis of Plasma and CSF

Enabling Researchers to Obtain Quantitative Proteomic Data from Just 11 µL of Sample with Only 5 Minutes of Prep Time

The newly released CodePlex Secretome solution uses one piece of equipment, the IsoLight, for a faster approach to obtaining highly multiplexed cytokine data. Requiring only 11 µL per sample and only five minutes of hands-on time, CodePlex provides an entirely automated workflow to increase work-away time. With IsoPlexis’ system, you can load your samples onto the CodePlex chip and walk away, achieving fully analyzed data the same day. Currently, CodePlex technology is being implemented in a consortium with the Institute for Systems Biology to identify SARS-CoV-2 prognostic immune biomarkers. To learn more about how this solution can be applied to studying the tumor microenvironment, read this post, and to learn more about its applications beyond cancer therapy, read this post.

CodePlex for Rapid Plasma and CSF Analysis

Researchers are using CodePlex technology for the rapid analysis of small amounts of patient plasma and CSF in various studies. This chip uses very small sample volumes to “sensitively sample a large panel of protein biomarkers over broad concentration ranges”1 to rapidly measure cytokines from patient samples.

One popular method for measuring protein biomarker levels is a protein sandwich assay or ELISA. While many microfluidic platforms require numerous instruments to prep, run, and analyze samples, researchers can now use IsoPlexis’ platform, which automates the laborious multi-step process of a traditional workflow into one, easy-to-use system.2 The Codeplex secretome solution can evaluate very small sample volumes of plasma or CSF quickly and efficiently in an automated manner.


On each CodePlex chip, there are individual barcode strips, each strip representing an assay for one protein, with the full barcode representing the full panel. Each individual barcode strip contains specific antibody-antigen binding that enables each plasma protein biomarker to be captured.2 In two cases, researchers used CodePlex technology to study plasma samples, one case studying two samples from the same healthy volunteer, using the highly multiplexed biomarker panel.1-2

These researchers were able to use the CodePlex Secretome technology to analyze their samples on a completely automated microfluidic platform—the IsoLight system—to assay dozens of plasma-based protein biomarkers “initiated with a simple push-button operation. This simple ‘plug-and-play’ platform can potentially permit high throughput, accurate, multiplexed [plasma] diagnostic measurements in remote locations and by minimally trained individuals.”2

Revealing True Functional Biology to Accelerate Research and Medicine

The IsoSpeak software family provides an application suite supporting each of our chips. This innovation creates an impactful end-to-end workflow and application for almost every key cell type, from early optimization to applying key, correlative insights and visualizations. Many researchers need to run both single-cell and population cytokine assays, so rather than purchasing multiple systems that require lots of space, time, and expertise to run, the IsoLight system serves as a proteomic hub that can automatically run both of these assays much faster and more efficiently than other systems currently available.

IsoPlexis’ CodePlex Secretome solution provides numerous clear benefits over conventional multiplexed bulk ELISA platforms and provides fully automated highly multiplexed proteomics with only five minutes of hands-on sample loading time. With the cases presented here as well as the ones discussed in previous posts, it’s easy to see the diversity this chip solution provides. From cancer therapy development to infectious disease research, it is easy to work this solution into any program or workflow.

Download our latest CodePlex Tech Note and CodePlex eBook to learn more about this innovative new solution. Speak to an expert today to see how CodePlex and IsoPlexis’ other solutions can fit into your existing workflow.


  1. Fan R, et al. Integrated barcode chips for rapid, multiplexed analysis of proteins in microliter quantities of blood. Nature Biotechnology 26: 1373-1378, 2008.
  2. Qin L, et al. Self-powered microfluidic chips for multiplexed protein assays from whole blood. Lab on a Chip 9: 2016-2020, 2009.
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