Single-Cell Proteomic Pathway Analysis for Integrated Cell Biology

  • Single-cell phosphoproteomics identifies functional adaptations of cancer cells that are undetected by genomic profiling, revealing adaptive resistance pathways that can become targets for the treatment of therapeutic resistance.
  • IsoPlexis’ Single-Cell Intracellular Proteome solution saves time compared to traditional methods, which can take months to analyze the interactions between just two pathways, and often require a data informaticist and other specialized personnel. IsoPlexis’ solution quantifies 15+ analytes at once, with lysing on-chip, and scientists obtain same-day publication-ready data automatically with IsoPlexis’ data informatics software, IsoSpeak.
  • The Single-Cell Intracellular Proteome solution was chosen as a Top 10 Innovation of 2020 by The Scientist for its ability to reveal true functional biology and generate predictive intracellular discoveries to accelerate research and medicine.

IsoPlexis’ Platform Identifies the Functional Adaptations and Protein Pathways that Genomics Misses

While researchers often look to genomic markers to understand the tumor microenvironment and cancer signaling pathways, functional adaptations can occur in the tumor that are not present in their genomic signatures. Specific protein interactions can be difficult to identify with genomics due to intermediate interacting partners and downstream effectors that confound the data. Thus, a critical goal of cancer research is to efficiently and accurately define the polyfunctional cells, or cells capable of changing their function, within therapeutic products. Through the identification of polyfunctional cells, researchers can characterize therapies that aren’t working as intended, and identify potential biomarkers that can become new druggable targets.  IsoPlexis’ Single-Cell Intracellular Proteome solution enables a comprehensive analysis of tumor signaling pathways and phosphorylation events that define signaling cascades directly from each single cell.

Chosen as a Top 10 Innovation of 2020 by The Scientist, The Single-Cell Intracellular Proteome solution uniquely measures cellular signaling via phosphorylation events and adaptive resistance pathways. The proteins are connected back to each individual cell to unlock the next level of resolution in cellular and immune research, enabling insights into intracellular pathways, disease progression, and mechanisms of therapy resistance. This solution reveals true functional biology to accelerate research and medicine. Within one week, researchers are able to generate publication-ready data shown to be highly correlative to in vivo biology in multiple publications.

Same-Day Sample-to-Answer Insights Accelerate the Development of Curative Medicines

With traditional technologies, obtaining tumor signaling data is time-consuming and difficult. Traditional methods are limited in the number of pathways that can be analyzed at once, adding significant time and resources and increasing research timelines to months or years. Once data is obtained via traditional methods, even with a trained data informaticist, characterization of the interactions between multiple pathways could add additional months.  While Western blot automates some workflow steps, such as incubation and detection, multiplexing abilities are limited, and assays involve long and expensive development processes for new analytes.

In contrast, IsoPlexis’ Single-Cell Intracellular Proteome solution enables analysis of 15+ analytes at once, with lysing on-chip. There’s no need for an informatics expert, as the IsoSpeak data informatics software automatically generates publication-ready data and advanced visualizations same-day. With IsoPlexis, researchers can run an entire multi-omic experiment, including single-cell phosphoproteome, metabolome, secreted proteome analyses, and highly multiplexed bulk proteomics, in one week.

What can the Single-Cell Intracellular Proteome solution do for your research? Talk with an expert now.

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