Single-Cell Analysis of Human Monocyte-Derived Dendritic Cells: How IsoPlexis’ Newest Protocol is Expanding Immune System Characterization

Given the functional heterogeneity of immune cells, analysis at the single-cell level can help to identify key individual cells driving immune response. IsoPlexis’ single-cell functional proteomics platform has helped to functionally characterize a variety of immune cells, including engineered cells, T cells, monocytes, NK cells, macrophages, and more. Now, IsoPlexis has developed a new protocol for the differentiation and functional phenotyping of human monocyte-derived dendritic cells to further investigate immune responses.

Dendritic cells are the most powerful antigen-presenting cells, circulating the body and acquiring antigens that can then be presented to prime T cells. Because of their important role in immune activation, characterizing dendritic cell function could lead to important insights for a many different diseases. Targeting dendritic cells can also lead to more effective therapies, and researchers are already exploring how dendritic cells can be harnessed to create better vaccines, treat cancer, improve organ transplantation, and improve multiple sclerosis therapies, along with many other applications.

Human Monocyte-Derived Dendritic Cell Protocol Overview

IsoPlexis’ newest protocol provides researchers with a validated workflow to differentiate and analyze human monocyte-derived dendritic cells. Starting with cryopreserved cells, the protocol details how to thaw and enrich samples for monocytes. Next, monocytes are differentiated to dendritic cells with GM-CSF and IL-4, a combination that has previously been shown to reliably generate dendritic cells. Cells are stained using CellTrace™ Far Red stain, stimulated with LPS, and loaded onto IsoCode chips for automated analysis by the IsoLight or IsoSpark instrument. For more details, the full protocol can be downloaded here.

As with all IsoPlexis protocols and workflows, the ELISA-based assay, imaging, data export, and data analysis steps are effortlessly automated, generating actionable results with minimal hands-on time – all on one instrument.

Expanding Single-Cell Applications to Accelerate Research

IsoPlexis’ IsoCode products are empowering researchers to better understand cell function at the single-cell level to advance research. The new Human Monocyte-Derived Dendritic Cell Protocol adds to the range of cell types that can be functionally phenotyped using IsoPlexis’ platform and can help researchers to gain an even deeper understanding of immune function. See IsoPlexis’ other applications here, or explore the Functional Cell Library to see how IsoPlexis’ single-cell functional analysis has been used to learn more about a variety of cell types and diseases.

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