IsoU E-Learning Series: Reducing Hands-On Experimental Time & Obtaining Quality Insights Faster with IsoPlexis’ Highly Multiplexed, Walk-Away Proteomics

  • IsoPlexis offers many resources, including webinars, eBooks, sample prep protocols, and expert roundtables to help researchers apply our unique functional proteomics technology to their experiments. The newly released high-plex automated immunoassay applications have several resources for users and prospective users as well. Researchers can access IsoUniversity (IsoU) to obtain product information and get started on training modules demonstrating things such as sample prep, analyzing data, and applying results obtained from IsoPlexis’ solutions.
  • A webinar with Dr. Denis Wirtz highlights how IsoPlexis’ CodePlex technology was used to identify the cytokines associated with enhanced motility, allowing researchers to have a potential method to prevent metastasis. The CodePlex eBook features this case and several others to provide insight into how researchers can apply IsoPlexis’ technology to make critical discoveries in cancer immunology and oncology, among other disciplines.

Webinar: How CodePlex Technology Uncovers Cytokine Drivers of Cancer Metastasis

In our previous post, we highlighted the numerous free resources offered by IsoPlexis for researchers using the intracellular signaling omics application, Single-Cell Intracellular Proteome, to make the most of their experiments. From on-demand webinars with leading researchers to eBooks showcasing how prominent research institutions have generated impactful data using our technology, these resources are on-hand to help researchers make the most of IsoPlexis’ unique platform. For each of our solutions, IsoUniversity offers training modules for preparing samples, executing runs, analyzing data, and applying results to identify biomarkers and predict patient outcomes. In today’s post, we’ll highlight some of the resources surrounding our new high-plex automated immunoassay application for highly multiplexed bulk proteomic analyses without the typical challenges associated with traditional bulk platforms.

IsoPlexis’ webinar with Dr. Denis Wirtz, the Vice Provost for Research and the Director of Johns Hopkins Physical Sciences-Oncology Center, discusses the role of cancer cell communication in promoting tumor cell migration and metastasis. Metastasis is responsible for 90% of deaths from solid tumors, while tumors that remain local can often be effectively treated or cured. With very few therapeutics available to stop the spread of cancer, metastasis poses a particular challenge to cancer treatment. Metastasis occurs when cancer cells switch from a proliferative to migrative phenotype. Researchers found that in a 2D plane, cancer cell proliferation led to enhanced motility, with a sudden increase in motility after a certain density threshold of cancer cells had been reached. The researchers hypothesized that at high density, cancer cells started secreting cytokines that promoted migration, and therefore metastasis. Using IsoPlexis’ high-plex automated immunoassay technology, CodePlex, the researchers identified two cytokines, IL-6 and IL-8, which increased in expression as density increased and were necessary for the onset of cancer cell migration. Further, CodePlex uncovered a novel IL-6 and IL-8 mediated synergistic paracrine signaling pathway, pointing to a potential therapeutic target against metastasis in cancer cells.

The next portion of the webinar discusses how CodePlex facilitates such critical research insights and highlights the many advantages of the IsoPlexis solution over status quo technologies. The CodePlex solution accelerates proteomic workflows and is the first fully automated highly multiplexed bulk cytokine solution. The sample prep for CodePlex is only five minutes of hands-on time, compared to the 6-10 hours required by traditional methods, and once you load the CodePlex chips, you insert them into the IsoLight or IsoSpark instrument and walk away, coming back later that same day to fully analyzed data. Additionally, IsoPlexis’ platform eliminates the need for multiple systems and automates steps such as vortexing, imaging, and data analysis, which typically require extensive training and specialized personnel. Another significant benefit of the CodePlex solution is its ability to highly multiplex 30+ cytokines from ultra-small sample sizes of 11 µL, using far less sample volume than the 100-200 µL required by other bulk platforms. This is a critical advantage when working with limited or difficult-to-obtain samples such as CSF. IsoPlexis’ CodePlex technology offers these numerous benefits over legacy technologies, with the same limit of detection of 5-5000 pg/mL, making this solution critical to functional phenotyping across multiple research areas.

Easy Walk-Away Workflows for Highly Multiplexed Bulk Proteomic Assays

Another crucial resource for researchers using the CodePlex solution is the sample prep protocol video, which can be found on the IsoUniversity section of our website, along with written protocols and educational resources for many other IsoPlexis solutions. All of IsoPlexis’ applications are backed by published data and established protocols to accelerate research timelines and workflows. IsoPlexis provides easy-to-follow protocols for everything from prepping your samples to executing the experiment so that that every lab can take advantage of this unique technology to increase productivity and accelerate research insights.

Additional Resources for Improving and Accelerating Bulk Proteomic Workflows and Quality Insights with IsoU

IsoPlexis’ unique applications including its CodePlex solutions for high-plex automated immunoassays are accessible to every lab regardless of size or budget on the IsoSpark, IsoLight, and IsoSpark Duo proteomics hubs. These fully automated, walk-away systems streamline and accelerate therapeutic timelines, returning publication ready data same day. Our CodePlex solution has been used by several scientists researching COVID-19. Watch our interview with Dr. Shelli Farhadian of Yale where she discusses using IsoPlexis’ bulk functional proteomics to identify key inflammatory biomarkers of neurological symptoms in COVID-19 patients and download our CodePlex eBook for additional case studies.

To learn more about the unique CodePlex technology and how to apply it to accelerate your lab’s research, watch our webinar launching this groundbreaking high-plex automated immunoassay application.

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