IsoPlexis’ single-cell barcode (SCBC) technology, now called IsoCode, was recently highlighted in a Nature Reviews publication, titled “Single-cell analysis targeting the proteome.” It is well known that cells are heterogeneous, and therefore, population-averaged measurements end up obscuring differences between cells and masking the presence of cell subsets with distinct characteristics. The high-need area of single-cell protein measurement tools “is extremely active and is producing many new systems that are becoming mainstream.”
Overcoming Challenges of Current Single-Cell Technologies
The publication highlighted how IsoPlexis’ technology is overcoming current challenges of traditional technologies: “although single-cell methods are mostly limited to the analysis of only a few samples at a time, barcoding techniques such as SCBC… are evolving to overcome this limitation.” IsoPlexis’ single-cell functional proteomics targets 3,200 to 16,000 cells across 8 chips in parallel and detects the true cytokine secretions from live single cells, up to 32+ cytokines per cell. IsoPlexis offers multiple panels and applications for the IsoCode chip solution, meaning that most cell types, from T cells, NK cells, CAR-T, monocytes, macrophages, fibroblasts, and more, can be analyzed. These solutions can, in a completely automated manner:
- Help researchers understand how to capture unique potency and durability metrics that correlate to in vivo
- Detect the most potent cell subsets, based on the ability of each cell to secrete multiple cytokine simultaneously, at a high intensity
- Translate single-cell data to therapeutic insights to help advance discovery and development programs
Highly Multiplexed Single-Cell Proteomics, Completely Automated
The IsoLight system can deliver highly multiplexed single-cell proteomic data in a hands-off manner on the same day. The publication continues, “As single-cell proteomics technologies continue to grow and mature, both the number of parameters that can be measured per cell and the number of cells analyzed will continue to increase. A technical bottleneck involves the level of multiplexing attainable using the current approaches, which is still not sufficient for interrogating more than a tiny part of the proteome.” This technology is now available globally with over 160 users obtaining highly-multiplexed and highly correlative single-cell proteomic data.
Single-cell functional data obtained via the IsoCode chip has correlated with patient response as well as persistence, suppression, and resistance within the tumor-immune environment. This highly multiplexed technology is currently being used by researchers all over the world to accelerate the discovery of novel therapeutics as well as accelerate the development of therapy programs. Single-Cell Polyfunctional Strength (human and mouse), Single-Cell Polyfunctional Inflammation, Single-Cell Innate & Myeloid, and Single-Cell Tumor Signaling (available late 2020) solutions from IsoPlexis cover a range of cell types, helping researchers detect the key cell subsets typically missed on other platforms that are necessary in accelerating therapies.
Fully Integrated Proteomics Workflow in One System
IsoPlexis’ completely integrated technology overcomes additional challenges that the authors pose: “Single-cell platforms still—for the most part—lack integrated instrumentation or consensus protocols for the generation of single-cell suspensions from tissues, particularly tumors, and the typical laboratory workflows are manual, low-throughput and not sufficiently reproducible for clinical applications.” Now for the first time, the complete single-cell workflow for highly multiplexed proteomics is available with IsoPlexis’ next generation proteomics platform.
The IsoLight system automates the numerous steps usually required to run multiplexed ELISA workflows. The automated end-to-end workflow is easy to incorporate into existing workflows. The award-winning user-friendly system is completely automated and hands-off. Just add your sample and walk away to achieve fully analyzed data on the same day—no need for any additional equipment such as washing and incubation stations. All of your proteomic needs are integrated into the completely automated IsoLight system.
Push-Button Informatics Software for Completely Automated Single-Cell Analysis
IsoPlexis’ award-winning IsoSpeak software suite overcomes another challenge: “another caveat is that the computational approaches needed for in-depth analysis of single-cell data are even less mature than the experimental platforms.” This state-of-the-art automated data analysis and visualization software was recently published in Proteomics with researchers at Kite Pharma.
IsoPlexis’ Edison Award-winning IsoSpeak software suite is the first automated informatics suite for advanced, functional, cellular mapping and visualizations which can reveal correlative insights into true, functional immune biology. IsoSpeak’s push-button user interface and advanced automation allows users to visualize, target, and utilize data from direct, functional cytokine profiling of single cells. The single-cell cytokine visualizations within the IsoSpeak software help stratify, correlate, and gather insight by revealing differences in each sample run. For more information about the advanced visualizations IsoSpeak offers, read our blog post here.