Given the functional heterogeneity of the brain’s immune landscape, understanding the role of each cell type and their interactions is essential to better understanding neurological disorders. IsoPlexis’ single-cell secretomics platform has helped to functionally phenotype immune cells implicated in a variety of neurological disorders and diseases, including the impacts of immune dysregulation in multiple sclerosis and immune cell response in Alzheimer’s disease. Now, IsoPlexis has released a new protocol for the functional phenotyping of mouse astrocytes to empower researchers with unique insights into how these cells facilitate and impact immune function in the brain.
Previously believed to be the support cells of the brain, astrocytes have since been revealed to be crucial for maintaining and restoring neurological function. Researchers have discovered the importance of astrocytes in several different functions, including impacting brain plasticity, supporting metabolic processes for neurons, and promoting the rehabilitation and repair of the brain following injury or disease. Understanding how astrocytes interact and facilitate these important processes is critical to identifying how neurological disorders and injuries progress for the development of new therapies.
Mouse Astrocyte Protocol Overview
IsoPlexis’ newest protocol provides researchers with a validated workflow analyze mouse astrocyte cells. First, cryopreserved cells are thawed and cultured. Next, the astrocytes are stimulated with LPS before staining. Following staining, the cells are lifted from the plates and loaded onto IsoCode chips for automated single-cell analysis by the IsoLight or IsoSpark instrument. The full protocol, IsoCode Single-Cell Adaptive Immune: Mouse Astrocytes Protocol, can be downloaded here.
As with all IsoPlexis protocols and workflows, the ELISA-based assay steps, imaging, data export, and data analysis are effortlessly automated, generating actionable results with minimal hands-on time – all on one instrument.
Accelerating Research with Advanced Single-Cell Applications
IsoPlexis’ IsoCode Single-Cell Secretome solution empowers researchers to better understand immune function at single-cell resolution to advance human health. The new Mouse Astrocyte Protocol adds to the range of cell types that can be functionally phenotyped using IsoPlexis’ platform and can help researchers uncover key insights into neurological processes. To see all of IsoPlexis’ cell profiling capabilities, check out our Application Protocols page which showcases all of the cell types and associated workflows that have been internally tested and validated on the IsoPlexis platform.