Neuroinflammation has been shown to play a key role in the pathogenesis of many neurological diseases. To uncover drivers of inflammation, it is critical to functionally characterize individual cells to better understand how cell subsets contribute to the immune response. For this reason, IsoPlexis has developed the IsoCode Single-Cell Secretome solution to functionally define each cell type in a heterogenous population. Researchers have used this highly multiplexed single-cell solution to gain unique insights into cytokine signatures for a variety of cell types, including T cells, NK cells, monocytes, macrophages, engineered cell therapies, and more, across a broad range of immunological applications.
Using MDMi Cells to Overcome Challenges of Researching Microglia
To better support neuroscience research, IsoPlexis has launched the new IsoCode Single-Cell Innate Immune: Human Monocyte-Derived Microglia-Like (MDMi) Cells Protocol that integrates seamlessly with existing IsoPlexis workflows. Microglia have been implicated in driving various neurological disorders, including Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis (ALS), but it is challenging to fully isolate these cells from the central nervous system (CNS).
To address this, researchers have turned to MDMi cells, which harness more easily attainable monocytes that can be differentiated into a microglia-like phenotype. MDMi cells have previously been used as a model to study the role of microglia in a number of diseases and share key characteristics of microglia, providing researchers a powerful tool for neuroscience research. By assessing the cytokine signatures of MDMi cells in a highly multiplexed manner, researchers can gain unique insights into the manifestations of microglia in neurological diseases by uncovering their role in hyperinflammation.
Human MDMi Protocol Overview: Prep, Run, Analyze
The MDMi protocol has been internally tested and validated to ensure generation of robust data. The protocol is comprised of thawing cryopreserved cells followed by immediately enriching for monocytes. Upon enrichment, monocytes are differentiated to MDMi and stimulated on a plate with LPS and IFN-ɣ for 24 hours. The stimulated MDMis are then stained, lifted from the plate, and loaded onto the IsoCode chip for analysis.
Just like all of IsoPlexis’ application protocols, the ELISA-based workflow is fully automated with our instruments, the IsoLight and IsoSpark. Just add your sample, walk away, and come back to same-day fully analyzed data with publication-ready visualizations.
The full IsoCode Single-Cell Innate Immune: Human Monocyte-Derived Microglia-Like (MDMi) Cells Protocol can be downloaded here.
Explore Hyperinflammation in the Functional Cell Library to Guide Your Neuroscience Experiments
IsoPlexis has developed a library of cells characterized by functional proteomics – the Functional Cell Library (FCL). The FCL adds a unique layer of published, peer-reviewed proteomic data on a wide range of immune cell types and is designed to provide researchers a database to explore how single-cell functional proteomics can fuel their research. The hyperinflammation section of the FCL highlights publications where highly polyfunctional cells promoted disease progression in vivo, including neuroscience case studies. Visit the Functional Cell Library to see how it can guide your new projects.