Breakthrough discoveries across numerous applications utilizing the IsoCode Chip (SCBC).

Cancer Immunotherapy and Immunopathogenesis

Xue Q, Bettini E, Paczkowski P, Ng C, Kaiser A, McConnell T, Kodrasi O, Quigley M, Heath JR, Fan R, Mackay S, Dudley M, Kassim S, and Zhou J. Single-Cell Proteomic Assessment of CD19 CAR-T Cells Reveals a Complex Landscape of Polyfunctional Antigen-Specific Response. Presented at FOCiS 2017.

Rossi J, Paczkowski P, Shen Y, Morse K, Flynn B, Kaiser A, Ng C, Gallatin K, Cain T, Fan R, Mackay S, Heath JR, Rosenberg SA, Kochenderfer JN, Zhou J, and Bot A. Polyfunctional Anti-CD19 CAR T Cells Determined by Single-Cell Multiplex Proteomics Associated with Clinical Activity in Patients with Advanced Non-Hodgkin’s Lymphoma. Presented at AACR 2017, Session MS.CL10.01 - Clinical Biomarkers.

Mackay S, Flynn B, Morse K, Paczkowski P, Bacchiocchi A, Fan R, Halaban R, and Zhou J. Single-Cell Cytokine Profiling of Tumor-Infiltrating T Cells to Measure Patient Responses to Anti-PD-1 Therapy. Journal of Clinical Oncology, 35 (9), Suppl. 7S (2017).

Xue Q, Bettini E, Paczkowski P, Ng C, Kaiser A, McConnell T, Kodrasi O, Quigley M, Heath JR, Fan R, Mackay S, Dudley M, Kassim SH, and Zhou J. Novel Multiplexing Technologies for Comprehensive Functional Characterizations of CAR-T Final Products. Presented at BioMAN Workshop 2016.

Kleppe M, Kwak M, Koppikar P, Riester M, Keller M, Bastian L, Hricik T, Bhagwat N, Abdel-Wahab OI, Marubayashi S, Chen JJ, Romanet V, Fridman JS, Bromberg J, Murakami M, Radimerski T, Michor F, Fan R, and Levine RL. JAK-STAT Pathway Activation in Malignant and Non-Malignant Cells Contributes to MPN Pathogenesis and Therapeutic Response. Cancer Discovery, 5 (3), 316-31 (2015).

Ma C, Cheung AF, Chodon T, Koya RC, Wu Z, Ng C, Avramis E, Cochran AJ, Witte ON, Baltimore D, Chmielowski B, Economou JS, Comin-Anduix B, Ribas A, and Heath JR. Multifunctional T Cell Analyses to Study Response and Progression in Adoptive Cell Transfer Immunotherapy. Cancer Discovery, 3, 418 (2013).

Ma C, Fan R, Ahmad H, Shi Q, Comin-Anduix B, Chodon T, Koya RC, Liu CC, Kwong GA, Radu CG, Ribas A, and Heath JR. A Clinical Microchip for Evaluation of Single Immune Cells Reveals High Functional Heterogeneity in Phenotypically Similar T Cells. Nature Medicine, 17, 738-743 (2011).

Cancer Biology and Targeted Therapy

Wei W, Shin YS, Xue M, Matsutani T, Masui K, Yang H, Ikegami S, Gu Y, Herrmann K, Johnson D, Ding X, Hwang K, Kim J, Zhou J, Su Y, Li X, Bonetti B, Chopra R, James CD, Cavenee WK, Cloughesy TF, Mischel PS, Heath JR, and Gini B. Single-Cell Phosphoproteomics Resolves Adaptive Signaling Dynamics and Informs Targeted Combination Therapy in Glioblastoma. Cancer Cell, 29:4 563-573 (2016).

Poovathingal SK, Kravchenko-Balasha N, Shin YS, Levine RD, and Heath JR. Critical Points in Tumorigenesis: A Carcinogen-Initiated Phase Transition Analyzed via Single-Cell Proteomics. Small, 12 (11), 1613-6829 (2016).

Kravchenko-Balasha N, Wang J, Remacle F, Levine RD, and Heath JR. Glioblastoma Cellular Architectures are Predicted Through the Characterization Of Two-Cell Interactions. Proc. Natl. Acad. Sci., 111, 6521- 26 (2014).

Wei W, Shi Q, Remacle F, Qin L, Shackelford DB, Shin YS, Mischel PS, Levine RD, and Heath JR. Hypoxia Induces a Phase Transition within a Kinase Signaling Network in Cancer Cells. Proc. Natl. Acad. Sci., 110 (15), (2013).

Wang J, Tham D, Wei W, Shin YS, Ma C, Ahmad H, Shi Q, Yu J, Levine RD, and Heath JR. Quantitating Cell-Cell Interaction Functions with Applications to Glioblastoma Multiforme Cancer Cells. Nano Letters, 12 (12), 6101-6106 (2012).


Zhou J, Kaiser A, Ng C, Karcher R, McConnell T, Paczkowski P, Fernandez C, Zhang M, Mackay S, and Tsuji M. CD8+ T-Cell Mediated Anti-Malaria Protection Induced by Malaria Vaccines; Assessment of Hepatic CD8+ T Cells by SCBC Assay. Human Vaccines & Immunotherapeutics, 13 (7), 1625-1629 (2017).

Xue Q, Lu Y, Eisele MR, Sulistijo E, Fan R, and Miller-Jensen K. Analysis of Single-Cell Secretion Reveals a Role for Paracrine Signaling in Coordinating Macrophage Response to TLR4 Stimulation. Science Signaling, 8, 381 (2015).

Lin L, Ma C, Wei B, Aziz N, Rajalingam R, Yusung S, Erlich HA, Trachtenberg EA, Targan SR, McGovern DPB, Heath JR, and Braun J. Human NK Cells Licensed by Killer Ig Receptor Genes have an Altered Cytokine Program that Modifies CD4+ T Cell Function. Journal of Immunology, 193, 940-9 (2014).

Zhao JL, Ma C, O'Connell RM, Mehta A, Diloreto R, Heath JR, and Baltimore D. Conversion of Danger Signals into Cytokine Signals by Hematopoietic Stem and Progenitor Cells for Regulation of Stress-Induced Hematopoiesis. Cell Stem Cell, 14(4), 445-459 (2014).

Xue M, Wei W, Su Y, Johnson D, and Heath JR. Supramolecular Probes for Assessing Glutamine Uptake Enable Semi-Quantitative Metabolic Models in Single Cells. Journal of the American Chemical Society, 138 (9), 3085-3093 (2016).

Xue M, Wei W, Su Y, Kim J, Shin YS, Mai WX, Nathanson DA, and Heath JR. Chemical Methods for the Simultaneous Quantitation of Metabolites and Proteins from Single Cells. Journal of the American Chemical Society, 137 (12), 4066–4069 (2015).

Lu Y, Xue Q, Eisele MR, Sulistijo E, Brower K, Han L, Amir ED, Pe’er D, Miller-Jensen K, and Fan R. Highly Multiplexed Profiling of Immune Effector Functions Reveals Deep Functional Heterogeneity in Response to Pathogenic Ligands. Proc. Natl. Acad. Sci., 112 (7), 607-615 (2015).

Elitas M, Brower K, Lu Y, Chen JJ, and Fan R. A Microchip Platform for Interrogating Tumor-Macrophage Paracrine Signaling at the Single-Cell Level. Lab on a Chip, 14, 3582 (2014).

Lu Y, Chen JJ, Mu L, Xue Q, Wu Y, Wu PH, Li J, Vortmeyer AO, Miller-Jensen K, Wirtz D, and Fan R. High-throughput Secretomic Analysis of Single Cells to Assess Functional Cellular Heterogeneity. Analytical Chemistry, 85 (4), 2548– 2556 (2013).

Shi QH, Qin LD, Fan R, Wei W, Guo DL, Shin YS, Hood L, Mischel P, and Heath JR. Single Cell Proteomic Chip for Profiling Intracellular Signaling Pathways in Single Tumor Cells. Proc. Natl. Acad. Sci., 109 (2), 419-424 (2012).

Shin YS, Remacle F, Fan R, Hwang K, Wei W, Ahmad H, Levine RD, and Heath JR. Protein Signaling Networks from Single Cell Fluctuations and Information Theory Profiling. Biophysical Journal, 100, 2378-2386 (2011).

Shin YS, Ahmad H, Shi Q, Kim H, Pascal TA, Fan R, Goddard III WA, and Heath JR. Chemistries for Patterning Robust DNA Microbarcodes Enable Multiplex Assays of Cytoplasm Proteins from Single Cancer Cells. Chem. Phys. Chem, 11 (14), 3063-3069 (2010).

Fan R, Vermesh O, Srivastava A, Yen BKH., Qin LD., Ahmad H, Kwong GA, Liu CC, Gould J, Hood L, and Heath JR. Integrated Barcode Chips for Rapid, Multiplexed Analysis of Proteins in Microliter Quantities of Blood. Nature Biotechnology 26, 1373-1378 (2008).

Heath JR, Ribas A, and Mischel PS. Single-Cell Analysis Tools for Drug Discovery and Development. Nature Reviews Drug Discovery, 15, 204-216 (2016).

Yu J, Zhou J, Sutherland A, Wei W, Shin YS, Xue M, and Heath JR. Microfluidics-Based Single-Cell Functional Proteomics for Fundamental and Applied Biomedical Applications. Annual Reviews of Analytical Chemistry, 7, 275-295 (2014).

Wei W, Shin YS, Ma C, Wang J, Elitas M, Fan R, and Heath JR. Microchip Platforms for Multiplex Single-Cell Functional Proteomics with Applications to Immunology and Cancer Research. Genome Medicine, 5, 75 (2013).