Improved Immune Monitoring and Correlates of Therapy Durability

Establish blood-based T cell biomarkers using IsoCode polyfunctional profiling to predict therapy durability and patient survival.

IsoCode Data

Figure 1: Single-cell analysis identifies a significant association of CD4+ PSI fold change (post/pre vaccination) with overall survival (OS) of patients with pancreatic cancer after GVAX treatment. (Zhou 2018).

Figure 2: PSI composition demonstrates that IFN-g followed by TNF-a were the major drivers for the enhanced PSI of CD4+ T cells in patients by GVAX treatment (Zhou 2018).

Publications

Lu Y, Xue Q, Eisele MR, Sulistijo E, Brower K, Han L, Amir ED, Pe’er D, Miller-Jensen K, and Fan R. Highly Multiplexed Profiling of Immune Effector Functions Reveals Deep Functional Heterogeneity in Response to Pathogenic Ligands. Proc. Natl. Acad. Sci., 112 (7), 607-615 (2015).

Lu Y, Chen JJ, Mu L, Xue Q, Wu Y, Wu PH, Li J, Vortmeyer AO, Miller-Jensen K, Wirtz D, and Fan R. High-throughput Secretomic Analysis of Single Cells to Assess Functional Cellular Heterogeneity. Analytical Chemistry, 85 (4), 2548– 2556 (2013).

Ma C, Fan R, Ahmad H, Shi Q, Comin-Anduix B, Chodon T, Koya RC, Liu CC, Kwong GA, Radu CG, Ribas A, and Heath JR. A Clinical Microchip for Evaluation of Single Immune Cells Reveals High Functional Heterogeneity in Phenotypically Similar T Cells. Nature Medicine, 17, 738-743 (2011).

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