Increased Sensitivity of Cell Therapy Product Manufacturing Analysis

Apply IsoCode's improved sensitivity to determine optimal CAR-T and TCR-T manufacturing processes and end products.

IsoCode Data

Figure 1: Comprehensive functional characterization of CAR-T cells (Xue 2016).

Figure 2: Polyfunctional heat map of anti-CAR bead stimulated CD4+ CAR-T cell data (Xue 2016).

Publications

Mackay S, Flynn B, Morse K, Ng C, Paczkowski P, Mahendravada A, Shinners N, Spencer D, Foster A, and Zhou J. Single-Cell Multiplex Proteomics Reveals Synergistic Impact of Antigen and Rimiducid-Dependent Stimulatory Signals on Promoting Polyfunctional GoCAR-T cells Targeting Prostate Stem Cell Antigen (PSCA). Presented at ASH Annual Meeting 2017.

Xue Q, Bettini E, Paczkowski P, Ng C, Kaiser A, McConnell T, Kodrasi O, Quigley M, Heath JR, Fan R, Mackay S, Dudley M, Kassim S, and Zhou J. Single-Cell Multiplexed Cytokine Profiling of CD19 CAR-T Cells Reveals a Diverse Landscape of Polyfunctional Antigen-Specific Response. Journal for ImmunoTherapy of Cancer, 5:85 (2017).

Xue Q, Bettini E, Paczkowski P, Ng C, Kaiser A, McConnell T, Kodrasi O, Quigley M, Heath JR, Fan R, Mackay S, Dudley M, Kassim S, and Zhou J. Single-Cell Proteomic Assessment of CD19 CAR-T Cells Reveals a Complex Landscape of Polyfunctional Antigen-Specific Response. Presented at FOCiS 2017.

Xue Q, Bettini E, Paczkowski P, Ng C, Kaiser A, McConnell T, Kodrasi O, Quigley M, Heath JR, Fan R, Mackay S, Dudley M, Kassim SH, and Zhou J. Novel Multiplexing Technologies for Comprehensive Functional Characterizations of CAR-T Final Products. Presented at BioMAN Workshop 2016.

Lu Y, Xue Q, Eisele MR, Sulistijo E, Brower K, Han L, Amir ED, Pe’er D, Miller-Jensen K, and Fan R. Highly Multiplexed Profiling of Immune Effector Functions Reveals Deep Functional Heterogeneity in Response to Pathogenic Ligands. Proc. Natl. Acad. Sci., 112 (7), 607-615 (2015).

Lu Y, Chen JJ, Mu L, Xue Q, Wu Y, Wu PH, Li J, Vortmeyer AO, Miller-Jensen K, Wirtz D, and Fan R. High-throughput Secretomic Analysis of Single Cells to Assess Functional Cellular Heterogeneity. Analytical Chemistry, 85 (4), 2548– 2556 (2013).

Ma C, Fan R, Ahmad H, Shi Q, Comin-Anduix B, Chodon T, Koya RC, Liu CC, Kwong GA, Radu CG, Ribas A, and Heath JR. A Clinical Microchip for Evaluation of Single Immune Cells Reveals High Functional Heterogeneity in Phenotypically Similar T Cells. Nature Medicine, 17, 738-743 (2011).

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