CAR-T Product Manufacturing Analysis
To ensure consistent manufacturing and improved outcomes, T-cell secretomic Precision Profiling™ can determine cellular and donor heterogeneity of CAR-T products. IsoPlexis' IsoCode (SCBC) technology, integrated with an automated bioinformatics platform, simultaneously measures a wide range of cytokines secreted by single CAR-T cells, providing the unmet need of precision profiling functional heterogeneity of CAR-T cell products. These novel biomarker capture and analysis methods have the potential to guide more sensitive CAR-T product pre-infusion quality assessments, which may enable CAR-T cells engineered with more efficacious and less immunotoxic profiles.
Mackay S, Flynn B, Morse K, Ng C, Paczkowski P, Mahendravada A, Shinners N, Spencer D, Foster A, and Zhou J. Single-Cell Multiplex Proteomics Reveals Synergistic Impact of Antigen and Rimiducid-Dependent Stimulatory Signals on Promoting Polyfunctional GoCAR-T cells Targeting Prostate Stem Cell Antigen (PSCA). Presented at ASH Annual Meeting 2017.
Xue Q, Bettini E, Paczkowski P, Ng C, Kaiser A, McConnell T, Kodrasi O, Quigley M, Heath JR, Fan R, Mackay S, Dudley M, Kassim S, and Zhou J. Single-Cell Multiplexed Cytokine Profiling of CD19 CAR-T Cells Reveals a Diverse Landscape of Polyfunctional Antigen-Specific Response. Journal for ImmunoTherapy of Cancer, 5:85 (2017).
Xue Q, Bettini E, Paczkowski P, Ng C, Kaiser A, McConnell T, Kodrasi O, Quigley M, Heath JR, Fan R, Mackay S, Dudley M, Kassim S, and Zhou J. Single-Cell Proteomic Assessment of CD19 CAR-T Cells Reveals a Complex Landscape of Polyfunctional Antigen-Specific Response. Presented at FOCiS 2017.
Xue Q, Bettini E, Paczkowski P, Ng C, Kaiser A, McConnell T, Kodrasi O, Quigley M, Heath JR, Fan R, Mackay S, Dudley M, Kassim SH, and Zhou J. Novel Multiplexing Technologies for Comprehensive Functional Characterizations of CAR-T Final Products. Presented at BioMAN Workshop 2016.
Lu Y, Xue Q, Eisele MR, Sulistijo E, Brower K, Han L, Amir ED, Pe’er D, Miller-Jensen K, and Fan R. Highly Multiplexed Profiling of Immune Effector Functions Reveals Deep Functional Heterogeneity in Response to Pathogenic Ligands. Proc. Natl. Acad. Sci., 112 (7), 607-615 (2015).
Lu Y, Chen JJ, Mu L, Xue Q, Wu Y, Wu PH, Li J, Vortmeyer AO, Miller-Jensen K, Wirtz D, and Fan R. High-throughput Secretomic Analysis of Single Cells to Assess Functional Cellular Heterogeneity. Analytical Chemistry, 85 (4), 2548– 2556 (2013).
Ma C, Fan R, Ahmad H, Shi Q, Comin-Anduix B, Chodon T, Koya RC, Liu CC, Kwong GA, Radu CG, Ribas A, and Heath JR. A Clinical Microchip for Evaluation of Single Immune Cells Reveals High Functional Heterogeneity in Phenotypically Similar T Cells. Nature Medicine, 17, 738-743 (2011).